FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790614

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790614
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7472698
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA385190.5154630897702543No Hit
CTTTCGTGTTCAAACCACGGAGTTCACAACACAGCAGCACACACAGCCGG234670.3140365099727033No Hit
GCCACTTGATTTCTTTTCCTTTCTCTCTCTACCAATATTAAGCCTTAGTG227070.3038661538309189No Hit
CAGCACATACACATTTTAGCATACCACAAATTCTTAACCCTTTCATATTC156920.2099910902327379No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAACTGTCT155170.2076492319106165No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAATTGCCT153060.2048256198765158No Hit
AGAAAGAATAAGCATGTTACCTTAAGATCAACCACCATGGACTGAACAAG146630.196220963298664No Hit
CCTGAAGCCACGGGTGTCTTAAGGCTTCTTCTGTCATAAAACGTGCCTTT105450.14111369146725855No Hit
CGGCGGCTGGTAAGGGAAGAGGGGCTCCTCACTTCCCAGTAGGGGCGGCC102770.13752730272252406No Hit
TAGATTAAACACTCAACAAAGTAAACATGTTTCTGTCCTCTGTCTCATGT96580.12924381528599177No Hit
CTGGGCTCATGTGACCTACTGCCTTGGCCTCCCAAAGTGCTGGGATTACA81740.10938485671440222No Hit
CAGTCAGCCTGCAGATGTGGATCTAATGATTGACTGCCTTGTTTCTTGCC77140.10322911483911165No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCGA17650.0106.8070764
GCTAGCC56650.0105.366351
CGTGTTC56950.099.305275
CGTGAGT8300.098.54791145
GGACGTA6550.098.351795
TCGTGTT59700.094.162974
CGCAATC6200.092.967283
CGACGTA5850.092.7329565
CGATACT6750.089.3830957
AAACGCG6350.088.608129
TAGCGGC25750.086.350688
CGGGTTA7700.084.543555
CCGATGT32600.084.244253
CACGGGT31950.083.173389
AGTCGAA23050.082.0792165
TAGCCCT74100.081.263893
TGACCGA34150.081.189869
CGCTCGA8100.081.1812749
GCCCGAT34150.080.044871
TAAGCGT6000.079.5227145
Produced by FastQC (version 0.11.5)