FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790616

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790616
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4691291
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA219410.4676964187469931No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAACTGTCT130200.27753554405386494No Hit
CTTTCGTGTTCAAACCACGGAGTTCACAACACAGCAGCACACACAGCCGG129810.2767042163873441No Hit
GCCACTTGATTTCTTTTCCTTTCTCTCTCTACCAATATTAAGCCTTAGTG114600.24428243739303318No Hit
AGAAAGAATAAGCATGTTACCTTAAGATCAACCACCATGGACTGAACAAG91790.19566042694857344No Hit
CGGCGGCTGGTAAGGGAAGAGGGGCTCCTCACTTCCCAGTAGGGGCGGCC77590.1653915734496112No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAATTGCCT67150.14313757129966995No Hit
CAGCACATACACATTTTAGCATACCACAAATTCTTAACCCTTTCATATTC66380.14149623206064174No Hit
CCTGAAGCCACGGGTGTCTTAAGGCTTCTTCTGTCATAAAACGTGCCTTT58120.1238891384056116No Hit
CTGGGCTCATGTGACCTACTGCCTTGGCCTCCCAAAGTGCTGGGATTACA56580.12060645992755513No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGAGT4750.0117.32657145
GCTAGCC31600.0104.8963851
TAGTCGA11700.0104.688444
CGTGTCG4850.0103.9639145
GGACGTA3800.0103.861965
CGCAATC4200.098.830883
AAACGCG4800.097.785949
CGACGTA3350.095.469615
CGGGTTA4100.094.602615
TCGTGTT38550.093.554274
CGCACGT5400.093.273141
CGTGTTC39650.090.787195
CGATACT4000.090.133137
AGTCGAA14400.086.004465
TAGCGGC17800.085.60458
TGACCGA22100.085.569919
GCCCGAT21850.085.041291
GTTCCGT5650.083.102245
CGCTCGA4800.082.196889
TGTCGAC13750.082.124739
Produced by FastQC (version 0.11.5)