FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790621

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790621
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3652188
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGCCAAGACTTCGGGGGGGGGGGGGGGGGGGGGGGGGGG122080.334265377357354No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAATT8450.087.821879
CGCGATT6700.084.637689
CGCTATT8250.080.6163569
CGCGATA7800.076.29189
GGCGATA8750.074.409519
GCGCATT9950.073.878799
TCGCATT8050.072.1831369
GACGTAT3500.070.008958
GATCGAT3800.070.008948
CGGTATA10050.069.6606459
AGCGATA11750.069.115229
GCGGATT11450.069.0918059
GTACGAT5950.068.244028
ACGCGAT4950.067.887478
TCGCATA8100.067.416029
ACGTTAT5300.066.046178
GCCGATT13400.065.829319
CGTAATT10450.065.654329
GCGCATA12150.064.5359
CGTTATA7000.064.008189
Produced by FastQC (version 0.11.5)