FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005790622

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790622
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4990806
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA235100.47106619652216497No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAACTGTCT133450.2673916798208546No Hit
AGAAAGAATAAGCATGTTACCTTAAGATCAACCACCATGGACTGAACAAG128930.2583350264466301No Hit
CTTTCGTGTTCAAACCACGGAGTTCACAACACAGCAGCACACACAGCCGG127560.2555899788531151No Hit
CGGCGGCTGGTAAGGGAAGAGGGGCTCCTCACTTCCCAGTAGGGGCGGCC125600.2516627574784514No Hit
GCCACTTGATTTCTTTTCCTTTCTCTCTCTACCAATATTAAGCCTTAGTG106900.21419385966915966No Hit
TACTAGTCGAAAGCGGCCCCGTGAAGGGGAAGCCGAGGGTGCCGAGACCA72380.1450266750500821No Hit
CTGGGCTCATGTGACCTACTGCCTTGGCCTCCCAAAGTGCTGGGATTACA62990.12621207877044308No Hit
CAGTCAGCCTGCAGATGTGGATCTAATGATTGACTGCCTTGTTTCTTGCC59370.11895874133356416No Hit
TGGCACACACTTCGAAGTTGAGGGGGGAATTCTGAGGAGGAACTGATGAT57930.1160734358338112No Hit
AAGATACTTATAGCTTCTTGTCTCCAGGTCTGTAGGTTTTTCTTTAAATT55730.11166533020918867No Hit
CAATGACAGCAGCTGACTTGACTTTGCTAATGCCAAACAGCAAGTAAAAT55210.11062341433427786No Hit
GCATAATTTGTTGGACTCTAAGATCAACACCCTGTTATCATTGTGCCAAG53970.10813884570949062No Hit
GGTACAGGCATCCTTCACCTGCTATTGTTGCAAGAACAGTCAGAATTTTA52420.10503313492850654No Hit
CAAAAGGAGAGCGTATCCAAGAGGCCTAGCAAGAGAATGAGAATCCGCAT52330.10485280333477197No Hit
AAAATTACGTTCTCTTTTCTAGTCGCCGAGATGACATGGAATCATTAGGA52000.1041915874910786No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAATTGCCT51830.10385096114735777No Hit
CTGAAAGGCACTGAAATCATTTGGCCTGTATCTTTTATGAGCACCATCTT51300.10278900842869869No Hit
GAATAAATCTGCGAAATCTGTACCTTATTCGCTTCCTTGTCAAAATATGA51200.10258863999121584No Hit
CCTGAAGCCACGGGTGTCTTAAGGCTTCTTCTGTCATAAAACGTGCCTTT50590.1013663925225705No Hit
CATTTGCTTTGCCATCTCCGCATTGTCCACTCACTTGCTCTGCCCTTCTG50390.10096565564760482No Hit
ACCTGACGGGTATCTTCATGCACGGCAAGATCCCCACGCTGAAGATCTCT50300.10078532405387026No Hit
GAAAATAAAACCCCAGAGGCAGAATTCCGAAGTTCAGCTGCATGCTGGTT50090.10036455033515627No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)