FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790628

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790628
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4163794
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA208920.5017539292289677No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAACTGTCT134460.32292663854167614No Hit
AGAAAGAATAAGCATGTTACCTTAAGATCAACCACCATGGACTGAACAAG82170.1973440568865799No Hit
GCCACTTGATTTCTTTTCCTTTCTCTCTCTACCAATATTAAGCCTTAGTG75840.1821415756879423No Hit
TACTAGTCGAAAGCGGCCCCGTGAAGGGGAAGCCGAGGGTGCCGAGACCA44900.10783434531103124No Hit
CTTTCGTGTTCAAACCACGGAGTTCACAACACAGCAGCACACACAGCCGG42300.10159004023734122No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGAGT5050.0120.513405145
CGCAATC3250.0111.3192753
CGACGTA5350.0108.9495545
GCTAGCC29400.0108.040081
TAGTCGA6800.0106.408134
GCTAGTC3650.0100.955453
GGACGTA4050.097.60165
TAATACG5350.096.426626
CGCACGT3900.096.222641
CGCTCGA4500.093.781039
CGATACT4300.090.355987
GCGCAAT4150.087.178792
TACTCGA6800.086.702924
GTTGCGA7000.086.139916
GCTAGAC5450.084.8238142
CGGGTTA4350.084.7097555
CGTACCC6950.082.921581
GACGTAC7150.081.521696
TAACGAA6700.080.5126145
AAACGCG5350.080.133329
Produced by FastQC (version 0.11.5)