FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790629

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790629
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4163794
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGCCAAGACTTCGGGGGGGGGGGGGGGGGGGGGGGGGGG98080.23555440062596758No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT7050.081.891759
GACGTAT3950.075.7220848
CGTTATA10250.073.291089
GTACGAT5600.072.042898
ACGTATA9400.069.558669
GCGAATT6250.067.8892449
GATCGAT4550.067.265518
GGCGATA7050.067.092039
GCGGATT10300.066.857359
CGCGATA8050.065.670289
CCGCATA10400.065.545669
GCGCTAT5950.064.297928
ACGTTAT6650.063.8056878
CGCGAAA9100.062.679239
CGCTATT9800.062.4608359
GCGCATT10450.061.2382439
TCGCATA9550.060.454129
GAACGAT5650.060.3252078
ACGCATT11250.058.7384229
GCGTATT11450.058.3199239
Produced by FastQC (version 0.11.5)