FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790637

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790637
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2063594
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGCCAAGACTTCGGGGGGGGGGGGGGGGGGGGGGGGGGG52340.2536351627306534No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT3200.079.389369
GGCGATA4850.074.205869
GACGTAT2400.073.506888
CGGGATA5300.073.231279
CGGTATA4350.073.00179
GCGAATT4000.070.568319
GTACGAT2700.070.5666058
CGCGTAT1300.070.56668
GCGGATT5700.069.330289
GCCGATT4950.067.0042659
TCGCATA4350.066.5126659
CGCTATT5250.065.863769
GATCGAT1950.065.1384058
ACGGATT6700.063.1955079
CGCCGAT3350.063.1939778
GCGCTAT3200.061.745788
ACGTATA4850.061.1107039
GTCGATA3350.061.088999
CGATATA6050.060.653769
ACCGATT6750.060.6364759
Produced by FastQC (version 0.11.5)