FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790638

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790638
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3988591
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA200970.5038621407910713No Hit
GCCACTTGATTTCTTTTCCTTTCTCTCTCTACCAATATTAAGCCTTAGTG96140.24103749920711348No Hit
AGAAAGAATAAGCATGTTACCTTAAGATCAACCACCATGGACTGAACAAG95250.23880613479797752No Hit
CTTTCGTGTTCAAACCACGGAGTTCACAACACAGCAGCACACACAGCCGG92820.23271375781572992No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAACTGTCT78550.19693671273890956No Hit
CGGCGGCTGGTAAGGGAAGAGGGGCTCCTCACTTCCCAGTAGGGGCGGCC70190.17597693019916055No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAATTGCCT60680.1521339239846853No Hit
CAGCACATACACATTTTAGCATACCACAAATTCTTAACCCTTTCATATTC51930.1301963525465509No Hit
CTGGGCTCATGTGACCTACTGCCTTGGCCTCCCAAAGTGCTGGGATTACA48200.12084467923635189No Hit
CAGTCAGCCTGCAGATGTGGATCTAATGATTGACTGCCTTGTTTCTTGCC42920.1076069218428262No Hit
TACTAGTCGAAAGCGGCCCCGTGAAGGGGAAGCCGAGGGTGCCGAGACCA42810.10733113523046107No Hit
ACCTGACGGGTATCTTCATGCACGGCAAGATCCCCACGCTGAAGATCTCT41790.10477384118852999No Hit
TGGCACACACTTCGAAGTTGAGGGGGGAATTCTGAGGAGGAACTGATGAT40130.1006119704928382No Hit
AAGATACTTATAGCTTCTTGTCTCCAGGTCTGTAGGTTTTTCTTTAAATT40030.10036125539068809No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGAGT4250.0128.887145
CGACGTA5100.0120.872095
TAGTCGA7800.0118.991634
CGCTCGA4200.0113.7733849
CGATACT4050.0112.857347
AAACGCG4800.0112.536719
CGCACGT5150.0108.967851
GGACGTA3200.0108.224665
GCTAGCC29900.0107.0511551
CGCGAGA3000.0106.20582
CGTACCC4800.0105.366421
TAATACG4850.0104.252716
TCGCGAG3150.0101.173751
GCTAGAC3800.096.605962
CGTGTCG3400.095.200615145
TGTCGAC15150.095.080529
CGCAATC2850.094.782023
CGGGTTA3950.094.689735
TGACCGA16800.093.986719
TCGTGTT28050.092.351714
Produced by FastQC (version 0.11.5)