FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790645

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790645
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1441852
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGCCAAGACTTCGGGGGGGGGGGGGGGGGGGGGGGGGGG34080.23636267800023858No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCTAT2700.086.19038
CGCGATT2550.083.4389
CGGTATA3850.079.442199
GACGTAT1350.078.802568
CGCGTAT1700.078.223148
ATCGTAT1550.077.2138068
CGCTATT4250.076.658669
CGCGATA3650.074.687029
GCGTATT3950.074.064439
CGTTATT3900.071.604259
CCGGTAT2800.071.238928
GGCGATA2900.071.075169
TCGCATA2650.070.253239
TCGCATT2850.069.989129
ACGCATA3800.069.989129
ACGTATA2850.069.989129
GCGGATT4850.069.916969
ACGTTAT2200.069.511928
CGACATT2800.066.489669
TAACGAT1500.066.489668
Produced by FastQC (version 0.11.5)