FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790646

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790646
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2366504
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA118310.4999357702332217No Hit
AGAAAGAATAAGCATGTTACCTTAAGATCAACCACCATGGACTGAACAAG57070.24115742039734564No Hit
CTTTCGTGTTCAAACCACGGAGTTCACAACACAGCAGCACACACAGCCGG57050.2410729075463215No Hit
GCCACTTGATTTCTTTTCCTTTCTCTCTCTACCAATATTAAGCCTTAGTG51760.21871925845044No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAACTGTCT51430.21732479640854188No Hit
CGGCGGCTGGTAAGGGAAGAGGGGCTCCTCACTTCCCAGTAGGGGCGGCC36550.1544472352465916No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAATTGCCT30430.12858630283320882No Hit
CTGGGCTCATGTGACCTACTGCCTTGGCCTCCCAAAGTGCTGGGATTACA28110.11878281211441012No Hit
TACTAGTCGAAAGCGGCCCCGTGAAGGGGAAGCCGAGGGTGCCGAGACCA27530.11633193943471043No Hit
CAGTCAGCCTGCAGATGTGGATCTAATGATTGACTGCCTTGTTTCTTGCC24520.10361275535557936No Hit
CCTGAAGCCACGGGTGTCTTAAGGCTTCTTCTGTCATAAAACGTGCCTTT24110.10188024190958478No Hit
TGGCACACACTTCGAAGTTGAGGGGGGAATTCTGAGGAGGAACTGATGAT23940.1011618826758797No Hit
CAGCACATACACATTTTAGCATACCACAAATTCTTAACCCTTTCATATTC23880.10090834412280732No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGAGT2350.0129.52307145
CGTGTCG2200.0121.05991145
CGCACGT2500.0118.922571
CGACGTA3250.0116.98075
TAGTCGA6250.0113.917924
GCTAGTC2000.0110.599933
CGATACT3100.0109.241257
TAATACG2600.0109.004746
CGCGAGA2000.0107.145942
AAACGCG2650.0106.929979
CGTACCC3800.0103.711541
CGGTTTA2700.0102.431151
GCTAGCC18200.099.912581
AACGGAC2650.099.122585
TGACCGA10800.096.630599
AGTCGAA7450.095.568735
CGTGTTC16250.094.4353265
CGCAATC2050.094.4145663
TTAGGCG3600.094.070849
GCTAGAC2650.093.907592
Produced by FastQC (version 0.11.5)