FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790653

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790653
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1946143
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGCCAAGACTTCGGGGGGGGGGGGGGGGGGGGGGGGGGG50320.25856270582377555No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT3850.085.774319
ACGCATA4400.079.843119
TCGCATA4300.078.431939
CCGCATA4350.077.530419
GTACGAT1750.076.2824258
CGCGTAT2700.075.4645848
GCGGATT5050.075.131589
GCGAATT4050.074.599099
CTCGATA4200.073.607749
ACGTTAT3450.073.314928
TCCGATT3950.071.151339
ACCGATA4100.070.261949
CGGTATA5400.070.261949
CGCATAT3450.069.241878
CGCGAAA4750.068.043149
GGCGATA4900.067.39419
CGTAATT5650.067.1539
CGCGATA4400.067.0682149
ATCGATA5500.065.151989
TACGCGA1200.064.405127
Produced by FastQC (version 0.11.5)