FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005790654

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790654
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1696359
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCACCCTAATGCCCTAATGTCNNNNNNNNNNNNN372482.1957616282874084No Hit
TCTCATACCTCCATTCCAAGCTTTCATNNNNNNNN236741.3955772333568544No Hit
CGGCGGCTGGTAAGGGAAGAGGGGCTCCTCACTTCCCAGTAGGGGCGGCC132670.7820868106338339No Hit
CTGTGTTCTTAGGCGGTATCACCNNNNNNNNNNNN131450.7748949367439322No Hit
AGATGTTTGGAGCTTTGGAGTGGTTCTGTATCNNN95610.5636189037815699No Hit
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA89000.5246530952469377No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN74960.44188759572708375No Hit
CAAAGATACCTGTTTCTGTTCTCTCTTACCCNNNN51740.3050061926750175No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAACTGTCT47340.2790682868425846No Hit
AGGTTACAGCACGGTCACCTTCGACGGGACGCCCAGCTACGGTCACACGC38340.22601347945806283No Hit
AGGTAAAATCAAGAGTCCACATATCAAGTAANNNN36590.21569726691107247No Hit
TTTGATATCTTCCCAGCAAAATAATCAGCTCTCATTTTCCCTTACAGAGT33010.19459324352922935No Hit
GCCACTTGATTTCTTTTCCTTTCTCTCTCTACCAATATTAAGCCTTAGTG31140.1835696335504454No Hit
GTGTGCCACGACAGCGATGAGAGTGACACTGCCAAGGCCGTGGAGGTGCA28750.16948063470055572No Hit
GCTACAGCCGCTCGAAGTCTCGGTCCCGCACTCGTTCTCGATCTCGGTCG27950.16476465182193156No Hit
GGCAGCAGTGTCGACGGCAGCGGCGGCGGCGGGTGGGAAATGGCGGAGTA27730.16346775653030993No Hit
GGATCAAGTGTTTGGTTTTGCACAGTCTCTTCTACACATCTCCGAGCCCA27500.16211191145270548No Hit
CAAACAGACGCTGGGCTATCGAAGCCCCGACGCCGGGCCACCGCACGCTT27360.16128661444894624No Hit
TAGCTACTGCCTGCCTTCGAAGAACGATGATTCAGAAGAAGAGGTGAGAG26410.15568638478058006No Hit
AGAAAGAATAAGCATGTTACCTTAAGATCAACCACCATGGACTGAACAAG26170.1542715899169928No Hit
CAGCTAACATCATACTTAGACTGCAAACTGGCCGGGGATGGTGCCTCACA25970.15309259419733676No Hit
GGTCTCCATCTGCTCTTTCACGGACATGCCCCCAATGCAGAGGGCGCAGC25590.15085250232999028No Hit
TCATGGTGACGGGAGCAGCCTCCGCTGGGAGGGAGCCCCGCCGGGCGGGG25040.1476102641009362No Hit
TGCTTGGTCTGCATAGCCTCGAGTTTGGGGCAGTCAGTGATCCGATGACC23880.14077208892693116No Hit
CCATTCTTATGTCTGGTTATATCTGCATTAGGTTATTGATGATGGTAGTA23750.14000574170915472No Hit
TACTAGTCGAAAGCGGCCCCGTGAAGGGGAAGCCGAGGGTGCCGAGACCA23610.1391804447053955No Hit
CCTTGGAGGCCCTTCAGTATGAGCTCTGCGGGCTCCTCCCAGCCACGGCC23440.13817829834368786No Hit
CGTTCGCCTCTTCACATCGGATTGGGTCTCACGCAAGGATGAGGCGGGGT23100.1361740056202726No Hit
AGATCTCTGACCCGTTCGGATCCTTTCCTCACTCGCCCACCATGGACAAC22990.13552555797446175No Hit
GACAGTTTTCAGAGACAGCTGATCGGTTGGAGCTGTTGCGCCGAGCAGTC22780.13428761246882293No Hit
CAAATAGGGTGTGATTTTGTTCTATATTCATCTTN22710.13387496396694332No Hit
GCAAGGTGACAAGCGAGAAAGCCTTTGCGGATGGCAGCGCAAACCTTAGC22620.13334441589309812No Hit
CCAAGTGGACTCTTTCAGGGAGACACATTTCGGATTACTAAAGCAGATGC22560.1329907171772013No Hit
CTTGTAGATTTTACTTTCCTCGTTGGTATTGCAGTGGCAGCAACAGAGCC22530.1328138678192529No Hit
CGTCTCCTCGCCGCGATCCTGGACTTCCTCTTGCTGCAGGACCCGGCTTC22440.13228331974540766No Hit
GCGTGCCCCTCACCAGTAGCTGCCGGCGCTGCCGTAACGGCACATAGGGC22300.13145802274164844No Hit
TGGAAATACCAGTCAAATGTCCATGGATCATGAAGAATTACTACGTACTC22240.13110432402575162No Hit
TGCTGTGACCAGGTCCTTGCTATGGGGGGGCAGCCCCTCTCCTGTGGCAG21960.12945373001823315No Hit
CTCAATCGGCTTGTTGTGATGCGTATCCCCGTAGATGCCAGCACGAGCCG21890.12904108151635355No Hit
GAGGTGAAGTCCATTGCTAATCAGATTCAAGAGGACTGGAAAAGTCTTCT21810.12856948322849113No Hit
GGGTTGCCCTGCGAGTCGAGGTGATTGAAGAAGACGTCCACCTCGTCTGG21740.12815683472661152No Hit
GCCGCCAAGGAGCGGGAGATCCATCGCAAGCTGCAAGCGTTCTGCGCAAG21740.12815683472661152No Hit
CCCACCCTAATGCCCTAATGTCTGTCTCTCTGTCCTAGGGCTCCCGGGGC21710.12797998536866312No Hit
CTGGGAGTGAGCGTCTTCTCCGACCAGGGCGGGAGGAAGTACATGGAGGA21300.12556304414336825No Hit
GGTACAGGCATCCTTCACCTGCTATTGTTGCAAGAACAGTCAGAATTTTA21260.12532724499943704No Hit
GCCTCAGCAAAGCTTCCCGTGTCCCCCAGGTTCGCTGACCACCTGCACAA20860.12296925356012495No Hit
TATGAAACCATTGCTTTTAGAATAATCATGGGCCAGACTGGGAAGAAATC20740.12226185612833132No Hit
CCCGTCCATGGCATCCATAGCGTCCTCAGCGTCGCGCTTGTCGTGAAAGC20620.12155445869653769No Hit
TCCTTCAGCGCCTCGGGTGGGATGATGGGGCCTGGGGGGCCCTGGGGACC20460.12061126212081286No Hit
CGGCGGCTGGTAAGGGAAGAGGGCATCTTCTGCGGCGTTTCGTCTGGCGG20270.11949121618713963No Hit
CCAGGTTCTTCTTGGTGGACAGGATCTTGGTGTAGTTGCACATCTTCCGC20240.11931436682919123No Hit
TCATGGTGACGGGAGCAGCCTCTACCAGCCTGTCCCAGGTGGTGAGCCCT20060.11825327068150078No Hit
CCCGGCTCTCATTCTAAGATCCAGGAGCACTCCTGCGGTGAAGGACAGGA20050.118194320895518No Hit
AGAGCTCAATTTCTTAGCATTTATAAAATAAGTACTCCAGTGTTATGTTT19950.11760482303568996No Hit
GCTCACGAACCCGCTTGCTGAAGGAGGAGAAGGGGTCCAGGCGCTCCTCG19930.11748692346372437No Hit
TTTTTCCCCTGTAATTAACCGCCAGCTCCTGCCCAGCAGCCCCCTCCCAG19840.11695637538987914No Hit
AAAAAAAGTCGGGGGAAAGTTAATTTTTAAACTTGCATTACTTTTATTTT19800.11672057624594794No Hit
TGGCGGTCTCCACCCTAGTTGGGTCCGACCACGAGCTCCAGGGACCTTGG19760.11648477710201673No Hit
CTTTCGTGTTCAAACCACGGAGTTCACAACACAGCAGCACACACAGCCGG19550.11524683159637789No Hit
CCTTCAGGTGAATGGGAAAGGACCGCTCCTGCTGGAGGGGCCCCAGCAGC19370.11418573544868746No Hit
TTGAATTTAAGTAATTTACTACCTTTCGTTCTGTAGCTCTATCATGTTCT19250.11347833801689383No Hit
TTTGGAATCCCCCATAATGCATTCTGTTTGCCCTTCCTTGTAGGGTCAGC19230.11336043844492821No Hit
TGGAGTTGAGTCCAATCTTGATACGNNNNNNNNNN19230.11336043844492821No Hit
CACAGGAGGGTGTGTAGGATGTGACACTCACCGGGGGGTGCTTGCGCTTC19190.113124639300997No Hit
CGGCGGCTGGTAAGGGAAGAGGGGCTCCTCACTTCCCAGTAGGGGCGGCT19120.1127119907991174No Hit
GCCGCGGGTTAGAAAATGTGACCTTGGCGACCCCAACGCCCCCGCCTCCC18960.11176879422339257No Hit
TCAAATTAAACAGTACAATTCAGTTAGCTGTGGAGTCTCTGAGTGGCAGC18930.11159194486544416No Hit
GCCCATGGGATCCTCATGCTTGAATGAGTGGTTGGGGAACTGCGCCGCAT18820.11094349721963336No Hit
GATCCAGACTCGGAACCGGAAGATGTCCAACAAGTCCAAGAAGAGCAAGA18690.11017715000185692No Hit
CCGCAGCCTCCGCCACATCTGCCGGCTTCTGGGGCCGAGCATCAGGGCCC18660.11000030064390852No Hit
CGAGCCCCTCTTCAGCTTGGTCAGTGGCCTAGTGAGCATGACCAACCCAC18620.10976450149997731No Hit
TCCCTTCCGGAATATGCAAGCTCTGGGTGGCCCAGCCCTGCCGCCAGACT18560.10941080278408048No Hit
GGAGTTGGCCGCAGACAAGAAGGGGAGGGAGCGGGAGAGGGAGGAGAGCT18450.10876235513826966No Hit
GGGCATCGGCATCGGCATGTCGGCGCGGTTCGCCGAGGTGTCGATGATGC18300.10787810834852764No Hit
GCAGCCTTGAGTCAACTGAGTTTAACTGTAAATGTTTTTTTAAATATTGG18250.10758335941861363No Hit
TCCTGTGGGAGCCTCAATGTTACCCTGGAACACCCCCTCTTCGTTGGAGG18090.1066401628428888No Hit
GCCCATGCCAACTGTTTAGCAACTTCAAGTTTCCATAATATATTTATACA17940.10575591605314677No Hit
ACATATACAAAAGGATAAACGTTAATCTGCCCTGTGCCTTTGCGTTAATT17830.10510746840733595No Hit
CATCCCTGCATGTACCATCCTAAGCAAGGGCAACTGCAGACTGTACAGAC17730.10451797054750792No Hit
GCAGAAGAAGATGGTCAGCCTAGACATCTGTCGCTACCTGGCCCTGGACG17650.1040463722596455No Hit
GAACAGGTCGGCCAGGAACTCGTCGTTGAAGGCGGCCGGGTCGATGTAGG17550.10345687439981749No Hit
CAGTCATCCCACTATGAAGGATCCTGTAAATGTGACCCCCAGTTCCACAC17510.10322107525588628No Hit
TATCAGACTAATTAATCTTCTTACTTCACACATTGGCTTTAATAATTGAA17450.10286737653998947No Hit
GCCTGACCTTAGTCTGCCATCTCTTCCTCCGCAGCCTACATGAAGCGGCG17260.10174733060631623No Hit
CCCGGTCCTCGACGTCCATCTGTTCCCTAGCCCATGGCGGTCACCATGCT17150.10109888296050541No Hit
CACTCACATTGTGCAGCTTGTAGTAGAGGCCACAGGCGTTGCAGACAGGG17030.10039148552871179No Hit
GGCTTGGTGATACTTTTGACCAGTGGAATGCTGTGCCTTCAGGGTTCTCG17000.10021463617076339No Hit
GCATAATTTGTTGGACTCTAAGATCAACACCCTGTTATCATTGTGCCAAG16990.10015568638478058No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)