FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790656

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790656
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2156586
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA119690.554997574870652No Hit
CGGCGGCTGGTAAGGGAAGAGGGGCTCCTCACTTCCCAGTAGGGGCGGCC58900.2731168615580366No Hit
CTTTCGTGTTCAAACCACGGAGTTCACAACACAGCAGCACACACAGCCGG55180.25586737556489747No Hit
GCCACTTGATTTCTTTTCCTTTCTCTCTCTACCAATATTAAGCCTTAGTG51280.2377832370237032No Hit
AGAAAGAATAAGCATGTTACCTTAAGATCAACCACCATGGACTGAACAAG50120.232404365047348No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAACTGTCT49640.230178624919201No Hit
TACTAGTCGAAAGCGGCCCCGTGAAGGGGAAGCCGAGGGTGCCGAGACCA35730.1656785307889414No Hit
CCTGAAGCCACGGGTGTCTTAAGGCTTCTTCTGTCATAAAACGTGCCTTT33150.15371517760015135No Hit
CTGGGCTCATGTGACCTACTGCCTTGGCCTCCCAAAGTGCTGGGATTACA26620.1234358379401517No Hit
CAGCACATACACATTTTAGCATACCACAAATTCTTAACCCTTTCATATTC25080.11629492169568012No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAATTGCCT23980.11119426723534329No Hit
CAGTCAGCCTGCAGATGTGGATCTAATGATTGACTGCCTTGTTTCTTGCC22350.10363602471684412No Hit
TAATAACTCCCAACTCACTGGTCCTCTAAACCTGCAGCAGGGAGATGGTG21700.10062200162664509No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGAGT2500.0137.80432145
TAGTCGA8900.0120.837584
CGACGTA2300.0113.974785
GACGTAA1950.0110.303026
CGCACGT2900.0106.6554261
CGATACT2550.0105.4220357
AGTCGAA10300.0105.0656365
GGACGTA2050.0104.922385
GCTAGCC16500.0103.915261
CGGTTTA2250.0101.606041
TAGCGGC6150.0100.5342948
CTAGTCG11300.095.172963
TAATACG2600.093.068186
GCCCGAT11850.091.9220661
TGACCGA11800.091.694959
CGTACCC3300.091.689941
TGTCGAC7150.091.1731959
CGGGTTA2250.089.62125
CCGATGT12000.089.62123
CGTTGCG3850.087.293375
Produced by FastQC (version 0.11.5)