FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790657

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790657
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2156586
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGCCAAGACTTCGGGGGGGGGGGGGGGGGGGGGGGGGGG58140.2695927730218039No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT3350.085.4937749
GATCGAT2200.079.3766258
GTACGAT3300.078.318278
GCGAATT4900.075.557099
ACCGATA4750.075.001869
CGGTATA5100.073.963779
GGCGATA4850.073.455429
GACGTAT2050.071.555128
TCCGATT3700.069.8546759
GCGCTAT2600.069.851438
CGTAATT6400.068.763199
GCGGATT6650.068.2799
CGCATAT4450.067.496898
CGCGATA5400.067.267469
CGCCGAT3350.066.7237558
GCGCATA5800.066.24159
GTCGATA3800.066.178119
ACGCATT5300.065.9006359
CGTGATT4400.065.091869
GCGCGAT2900.065.034098
Produced by FastQC (version 0.11.5)