FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790658

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790658
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1643198
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA83030.5052951622385129No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAACTGTCT34870.2122081453361068No Hit
AGAAAGAATAAGCATGTTACCTTAAGATCAACCACCATGGACTGAACAAG32760.19936733126500886No Hit
GCCACTTGATTTCTTTTCCTTTCTCTCTCTACCAATATTAAGCCTTAGTG24330.14806493191934267No Hit
CTTTCGTGTTCAAACCACGGAGTTCACAACACAGCAGCACACACAGCCGG20890.12713014499774222No Hit
TACTAGTCGAAAGCGGCCCCGTGAAGGGGAAGCCGAGGGTGCCGAGACCA16700.10163108767172306No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGTA2100.0106.919475
GCTAGCC13200.0106.60431
CGCAATC1950.0104.984293
AAACGCG1900.0104.24919
CGATACT1900.0104.24917
CGTGAGT1900.096.05158145
GCTAGTC1500.092.45393
TAGTCGA3350.090.6797264
TAGCCCT15550.090.4578863
GGACGTA1100.090.0525055
CTCAACG807.415201E-787.73943145
GCGCAAT2400.085.299732
CGTGTTC8750.081.510385
CGGATCT2200.081.029997
AGCGGTT3350.079.620255145
TCGTGTT9200.077.523464
CGGGTTA1200.077.0449145
CATACCG1900.076.449349
TAATACG2250.076.311166
CGCTCGA1500.074.827699
Produced by FastQC (version 0.11.5)