FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790660

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790660
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1599772
Sequences flagged as poor quality0
Sequence length35-151
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCGGCTGGTAAGGGAAGAGGGGCTCCTCACTTCCCAGTAGGGGCGGCC117510.734542172259547No Hit
CGAAGTGCGGAAGATGTGCAACTACACCAAGATCCTGTCCACCAAGAAGA61190.3824920051107283No Hit
CCAGGTTCTTCTTGGTGGACAGGATCTTGGTGTAGTTGCACATCTTCCGC48610.3038557994514218No Hit
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA48480.3030431836536706No Hit
TCATGGTGACGGGAGCAGCCTCCGCTGGGAGGGAGCCCCGCCGGGCGGGG47900.29941766701755No Hit
TCCACGTTGCGCTGCTTGGCCTTGTCGCGGCTCTTGCGCACCGCGATGTT46370.2898538041670938No Hit
GCCACTTGATTTCTTTTCCTTTCTCTCTCTACCAATATTAAGCCTTAGTG45330.2833528777850844No Hit
GAACAGGTCGGCCAGGAACTCGTCGTTGAAGGCGGCCGGGTCGATGTAGG45270.28297782433996843No Hit
CAGCCGCTCACTCCGTCTCAATATGTCTCAAGATGGCGGCCAATGTGGGA44990.28122757492942746No Hit
GCCAAGAAGTCGGTGGACAAGAACAGCAACGAGTACCGGGTGCGGCGCGA43530.2721012744316065No Hit
CAAGAAGAAGGCCGCGAACTTGAACAGCATCATCCACCGCCTGGAGAAGG41970.26234988485859234No Hit
AGGCCGCCCTAGAGCATTCTAAGCCGCGGGACCCGGGCACCCCCCGACAT40880.2555364139389863No Hit
CAAACAGACGCTGGGCTATCGAAGCCCCGACGCCGGGCCACCGCACGCTT39850.2490979964644962No Hit
GGCAGCAGTGTCGACGGCAGCGGCGGCGGCGGGTGGGAAATGGCGGAGTA39840.24903548755697688No Hit
CCTACTCACTTCCGCTCCGTTCTCTTGCCCGCCCTGCAGCGGCACCCGAC39170.24484739075318232No Hit
CGCTTCCCACGTGTCAGTTCCCTAGGCCCCAGGTGCAGACGCCCAGACCC38610.24134689193210032No Hit
CCTTCAGGTGAATGGGAAAGGACCGCTCCTGCTGGAGGGGCCCCAGCAGC38290.23934660689148204No Hit
CGGCGGCTGGTAAGGGAAGAGGCCGGCCAGCGCCAGCTGCTTGGCTTCAT37700.23565858134784204No Hit
TGGAGCTGACCAGTGACAATGACCGCCTGCGCAAGCGGGTGGAACAGCTG36490.22809500353800416No Hit
AACCTGAGAACTTCCTGATCTCTCTCGCTGTGAGACATGTCTGAGACTCC36410.2275949322778496No Hit
CCTCCCGAACCAGAGCGACGTGGTGGAGGCCGAGGGCAGCCACAGCAACT36390.22746991446281095No Hit
TGGCGGTCTCCACCCTAGTTGGGTCCGACCACGAGCTCCAGGGACCTTGG35880.22428196017932556No Hit
GCCGCGGGTTAGAAAATGTGACCTTGGCGACCCCAACGCCCCCGCCTCCC34740.21715594472212293No Hit
CCCCACCCACGTGTCTTTCAGCCCGGCCGCACCACCTGGGTCTCCGCCAT34600.21628082001685242No Hit
CGTCTCCTCGCCGCGATCCTGGACTTCCTCTTGCTGCAGGACCCGGCTTC34450.21534318640406258No Hit
AGATCTCTGACCCGTTCGGATCCTTTCCTCACTCGCCCACCATGGACAAC33910.21196770539801923No Hit
GCTACAGCCGCTCGAAGTCTCGGTCCCGCACTCGTTCTCGATCTCGGTCG32620.20390405632802672No Hit
TAGCGCTGGAAGAGGTCGGTGAACTGCAGCGACACCAGCTCGGCGCGCAG32160.20102864658213798No Hit
GGGTTGCCCTGCGAGTCGAGGTGATTGAAGAAGACGTCCACCTCGTCTGG31930.1995909417091936No Hit
CCCGTCCATGGCATCCATAGCGTCCTCAGCGTCGCGCTTGTCGTGAAAGC31720.19827825465128782No Hit
CCCGGCTCTCATTCTAAGATCCAGGAGCACTCCTGCGGTGAAGGACAGGA31330.19584040725803428No Hit
AGAAAGAATAAGCATGTTACCTTAAGATCAACCACCATGGACTGAACAAG31090.19434019347757056No Hit
GCATGCCACGGTCCCAACGGGCCCCCGGGGCTCTGCTTTCGAGAACCCGG30990.19371510440237732No Hit
CTCTGCTCCGTGCTGCCTACGCACTGGCGCTGCAACAAGACCCTGCCCAT30540.1909022035640079No Hit
GGGCCAGTACCTTGAAAGCGATGGGCAGGGTCTTGTTGCAGCGCCAGTGC30380.18990206104369875No Hit
TCCGACCCCCGCATGCACTATCCAGGCGCCTTCACCTACTCCCCGACGCC30180.18865188289331228No Hit
CCACCTGTACTACGGCGCCTCGGCCGGCTCCTACCAGTTCTCCATGGTGG28920.1807757605458778No Hit
GGGTGTCCTAGAGCGGAGAGTCCCTGGCGCCACTGCCCCGCGCGTAGGGG28610.17883798441277882No Hit
CTTTCGTGTTCAAACCACGGAGTTCACAACACAGCAGCACACACAGCCGG28480.17802536861502766No Hit
CTGCAGTTCCAGATCGCGCACTGCGGCCAGACCACCATGCACCTGCAGCC28090.1755875212217741No Hit
TCCCTTCCGGAATATGCAAGCTCTGGGTGGCCCAGCCCTGCCGCCAGACT27520.17202451349317277No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAACTGTCT27440.1715244422330182No Hit
AGACAATCAAAAACAGGCAACAACTTCGTGAAGAAGAGGCGCGGGCGTCC27180.16989921063751584No Hit
GGAGTTGGCCGCAGACAAGAAGGGGAGGGAGCGGGAGAGGGAGGAGAGCT27170.16983670172999651No Hit
CGCGTGGTGGACTCGACTTCGGAGCTGGTCAGCGTGGAGCAGACGCTGCT26680.16677376526154977No Hit
CGACACCGAGGAGCCCAAGTCTCAGGGAGAGGCCGAGCGGGAGGAGGTGC26330.16458595349837352No Hit
CTCAATCGGCTTGTTGTGATGCGTATCCCCGTAGATGCCAGCACGAGCCG25190.15745993804117087No Hit
CATCATCTCCTCCAGCTTAGGGTAGTTGTCCATGGTGGGCGAGTGAGGAA24720.15452201938776278No Hit
GACAGTTTTCAGAGACAGCTGATCGGTTGGAGCTGTTGCGCCGAGCAGTC24700.15439700157272412No Hit
GCTCCGCCTCGTAGAAGTCGGCCGACTCCATGGGGGAGTTAGAGTTCTCC24690.1543344926652048No Hit
CCTCTCGCTGCTTCACTTCACGGGGCGAACATGGCGCACAGCTGTCGGTG24230.151459082919316No Hit
TACTTCTCGAAGACGCGCCTCAGCGTGTCGGGCGAGGTGCGGTAGGTCAG24030.15020890476892956No Hit
CAATCAACTACAACACTTTCTAGCAGACATAAGGTAAAAATGGCATGGCT23840.14902123552606247No Hit
GACTTGGAGGTCGACCGAGATCGAGAACGAGTGCGGGACCGAGACTTCGA23780.14864618208094654No Hit
CAGCTAACATCATACTTAGACTGCAAACTGGCCGGGGATGGTGCCTCACA23740.14839614645086927No Hit
CGCACGTCGGAGCCCATTTGCTGAGGCTCAGACCCGGACGCCCCGCGGCT22920.1432704160342849No Hit
GTCCCTGGAGCTCGTGGTCGGACCCAACTAGGGTGGAGACCGCCACCGAG22910.14320790712676557No Hit
CGGGCCGGGTTCTCGAAAGCAGAGCCCCGGGGGCCCGTTGGGACCGTGGC22610.1413326399011859No Hit
AGGCATACTTCACTCTTTTCAAACCCGGAGGGCTGCGGAGATCCTCCCAC21910.13695701637483343No Hit
CTGGGAGTGAGCGTCTTCTCCGACCAGGGCGGGAGGAAGTACATGGAGGA21780.13614440057708221No Hit
CCGGCCTTTCACCTTGTCCATGAGCTTCCAGCACTTCTCCACCATCTTCT21760.1360193827620436No Hit
GCAAGGTGACAAGCGAGAAAGCCTTTGCGGATGGCAGCGCAAACCTTAGC21750.1359568738545243No Hit
CCCCCGAGCAGTACGAGGTCTACATGTACCAGGAGGTGGACACCATCGAG21710.13570683822444699No Hit
GCTGTTCGCCCGGGAGCATCCGCAGCACGCGCCGCTGCGCGCCGAGCTGG21680.13551931150188903No Hit
GGCTCCGGCTACGCACAACATCCACCTGGCAGAGTCCCGGGGCTGTCCCG21170.13233135721840364No Hit
TCATGGTGACGGGAGCAGCCTCTACCAGCCTGTCCCAGGTGGTGAGCCCT21140.13214383049584566No Hit
CGCACGTCGGAGCCCATTTGCTGCGGCTCAGACCCGGACGCCCCGCGGCT20950.13095616125297854No Hit
GAGGCCAGCGCTGAACGTCTCCAGGGCCGGAGGAGCCGCGGGGCGTCCGG20770.12983100091763078No Hit
CACACACACACACACAGTTTATTGCATTGTTAGATTTTATACATAAAATT20720.12951845638003415No Hit
GGGTTCGGGTGGCCTGATTGGGCTCATGAAGGACGCCTTCCAGCCGCACC20480.12801824259957043No Hit
CGCCATGCCGGGAGAACTCTAACTCCCCCATGGAGTCGGCCGACTTCTAC20170.12608046646647147No Hit
GGGCCGCCACTCAGAGCTATGAGCTACGGCCGCCCCCCTCCCGATGTGGA20120.12576792192887487No Hit
CAGCACATACACATTTTAGCATACCACAAATTCTTAACCCTTTCATATTC19970.12483028831608504No Hit
CCGGTGGGTTGGTCATGCTCACTAGGCCACTGACCAAGCTGAAGAGGGGC19950.1247052705010464No Hit
AAGGCACAGAAGGAGAAACTGAGGGAAATCCAGCTTCGGAGCGCAGGGGA19750.12345509235065996No Hit
GGGCCGAAGTCTGACCCTTTTTGTCTCCTAGCCTGGATCTCCTTGGTGAC19540.12214240529275422No Hit
CCCCTGCTGCTGTGGAAACAGGTAGTCGGGGATCATGGGAACCTGGAAGC19460.12164233403259965No Hit
GCCGGCTGTCATCACATAGGGACAGGGCTCTCCCTCTCCCAGGCCAGAGG19400.1212672805874837No Hit
CAATCATTTAAGAACAAAACTAGTTTATTTGGATTTGGGACTCTGAACAG19240.12026713806717457No Hit
GGTTCGGGCTCCACAACGATTTGAGTAACGTCCTCCATGTACTTCCTCCC19210.12007961134461662No Hit
ACAGAGTCTGGCCATGTTACCCAATCTGATCTTGAACTCCTGCTGTCATC19100.11939201336190408No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAATTGCCT18920.11826685302655628No Hit
CGGCGGCTGGTAAGGGAAGAGGGGCTCCTCACTTCCCAGTAGGGGCGGCT18920.11826685302655628No Hit
CTGGGCTCATGTGACCTACTGCCTTGGCCTCCCAAAGTGCTGGGATTACA18910.11820434411903696No Hit
CGCCTTCGTTCGCTTTCACGACAAGCGCGACGCTGAGGACGCTATGGATG18890.11807932630399831No Hit
CGAGCCCCTCTTCAGCTTGGTCAGTGGCCTAGTGAGCATGACCAACCCAC18790.1174542372288051No Hit
CGTCTCCTCGCCGCGATCCTTTCTGGCGAGTCCCCGTGCGGAGTCGGAGA18640.11651660361601528No Hit
GGGCATCGGCATCGGCATGTCGGCGCGGTTCGCCGAGGTGTCGATGATGC18260.11414126513028106No Hit
GGCCCCTCAGAACTCCCATTCGATAGGTTCCTCCCGGCTGGCGGCCTTCT18050.11282857807237531No Hit
GCCCGATGTGACCGAGCCCAGCGGAGCCTGAGCAAGGAGCGGGTCCGTCG17980.11239101571974006No Hit
ACCAGCTGGAAAGCCATCACACCTCCCCAGCCCGCGCTCTCCCCAGGGTG17980.11239101571974006No Hit
AGGTTACAGCACGGTCACCTTCGACGGGACGCCCAGCTACGGTCACACGC17960.11226599790470143No Hit
GAGCACGAGACGTCCATCGACATCAGCGCCTACATCGACCCGGCCGCCTT17640.11026571286408313No Hit
ACGATGACGAAGACGAAGACGAGGCGGACGACGAGGAGGCTGCTGAGGCG17640.11026571286408313No Hit
ACCGTCCGCCGTCTGCCAAGCCGCGCCTCCAGCGCCGGGTGCCGGTAGCC17410.10882800799113874No Hit
ACCAGCTGGAAAGCCATCACACCTCCCCAGCCCGCGCTCTCCCCTGGGTG17270.10795288328586823No Hit
GCTGAGGTAGCGGCGTCCTCGGGGGCCGCGGGCGCGGCGGTGGCGGTCGC17220.10764033874827164No Hit
CCACTCACCTGCGCTCTGAAATAGAAGAACAGGGCGACGCTGCAGACAAC17160.1072652853031557No Hit
GGGTTTCATCAAGAAGCAGAAGGGTTTCACCTCGTCCGAGCCGGCTAAGG17080.10676521404300113No Hit
CTTTGCAGTAGGTCAAGTTAGGATGAGAAGCATGTCCCCAGCTGAAGGGG17060.10664019622796249No Hit
GGGCATCGGCATCGGCATGTCGGCCATGGGCTCGGCCACGCGCTACCACA16990.10620263387532723No Hit
CAGACCACGTGTCCACCGCTCCAGCCCGTCGCTCAGGCCCGCCGCGGGCC16940.10589008933773064No Hit
GCCTGACCTTAGTCTGCCATCTCTTCCTCCGCAGCCTACATGAAGCGGCG16920.10576507152269199No Hit
GGCTCCGCAGAGGGTCGAGACCCCGGACCTGGGGGTTCCTCGCCCCCTTA16680.10426485774222827No Hit
CGTTCGCCTCTTCACATCGGATTGGGTCTCACGCAAGGATGAGGCGGGGT16650.1040773310196703No Hit
CGGTCAGGTGCTCGTAGGGCTGCTCGCCCGTGTCCGCCTGAGGGTTGAAG16650.1040773310196703No Hit
CCGCCTTCAACGACGAGTTCCTGGCCGACCTGTTCCAGCACAGCCGGCAG16600.1037647864820737No Hit
GCGCCCTTAGTGGCCTCCATCAGCCACCAAATGGTGGCTCTGCAGACCTT16590.10370227757455439No Hit
GCTGTTTGCAGGGTCCTAACTCAATCGGCTTGTTGTGATGCGTATCCCCG16570.10357725975951573No Hit
CCGTCAGTGACACCTGGTACTTGACGCCGTCCTTGTCCTCTCCTCGGGCT16540.10338973303695775No Hit
CGCGAGGAGGATGAAGCCAAGCAGCTGGCGCTGGCCGGCCTCTTCCCTTA16510.10320220631439982No Hit
CACAGGAGGGTGTGTAGGATGTGACACTCACCGGGGGGTGCTTGCGCTTC16380.10238959051664862No Hit
CGCACCCGGTACTCGTTGCTGTTCTTGTCCACCGACTTCTTGGCCTTGCC16330.10207704597905201No Hit
GCGCATCCTTAAGAGAGCCACCTGCAGCGCGAGGCGCGCCGCTCCAGGCG16250.10157697471889744No Hit
TTTTTAAGATAACTTTACTCACTTTTTCTATACTTGCTTGCATTTTTGCT16230.10145195690385879No Hit
CTCCATGCCCATCCTATCTGTGGATCCCGGGTGGAACCTCAGCTCCAGAA16060.10038930547603034No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAT1000.0146.04936145
CTCAACG1650.0141.62364145
CGCACGT6350.0128.056611
TAGCGGC12800.0125.954048
CGTTGCG8700.0121.4875955
TAGTCGA4800.0119.9787454
AGTCGAA5050.0114.039195
TGACCGA10750.0110.274419
CGGGTTA5450.0109.398855
TGTCGAC9900.0108.7945259
GCCCGAT10900.0108.794271
CGATACT1250.0108.383977
GCTGGTA40750.0107.9055256
GTAGCGG15400.0105.128947
CGACGTA1500.0103.8874745
AGTGTCG10450.0103.0685047
TGGTAAG42450.0102.766918
CTAGTCG5650.0101.928843
CTGGTAA43350.0100.7896047
CGTCGGA9200.0100.156165
Produced by FastQC (version 0.11.5)