FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790661

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790661
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1599772
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGCCAAGACTTCGGGGGGGGGGGGGGGGGGGGGGGGGGG39780.24866043411186095No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT3500.091.973179
CGCGATT3250.083.975519
CGTGATT3450.079.107369
GTCGATA3750.074.644899
ACGGATT3700.073.762279
ACGTTAT2000.073.4762658
TCGCATA3650.072.855479
CGGTATA3850.072.706069
GACGTAT1650.072.097928
ACCGATA3350.071.024069
CGCGATA3600.069.979599
CGCGTAT2000.069.97748
GCGGATA5250.069.313129
GCGAATT3350.068.935129
CGTTATA4200.068.3134169
GCGCATT4000.068.2300959
CGTAATT3900.068.185249
ACGTATT5450.066.7695169
ACGCATT3150.066.6472249
CACGATA4350.065.957789
Produced by FastQC (version 0.11.5)