FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790663

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790663
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1835789
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGCCAAGACTTCGGGGGGGGGGGGGGGGGGGGGGGGGGG44010.239733433417457No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT1950.092.7200558
ACGTTAT2950.089.5769968
GCGAATT5000.080.6664359
CGCGATT3300.080.07649
GACGTAT2400.078.2325448
ACGCGTA1550.076.27187
GCGGATT5500.075.861869
GCGTATT5250.074.176059
CCGCATA3900.071.323129
CGATATA4900.070.959229
CGTGATT5600.069.540049
CGTCTAA2700.069.540048
CGCCGAT1900.069.540048
CGTAATT5850.068.945689
ACGCATA4150.068.70229
GTCGATA3850.068.6369259
GCGCATT5000.068.149239
CGACATT3800.067.7100459
GATCGAT1750.067.5531858
ACCGATA3950.066.899289
Produced by FastQC (version 0.11.5)