FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790667

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790667
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1795086
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGCCAAGACTTCGGGGGGGGGGGGGGGGGGGGGGGGGGG40320.2246131940196737No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT3550.083.90299
ACGTATA3600.082.737589
GACGTAT1700.079.640458
GCGAATT4550.077.365019
CGCGATA3950.077.120189
GTACGAT2200.076.925448
ACGCGAT2600.070.298018
GCGCATA5950.069.400969
TCGCATA4300.069.268679
ATCGTAT2600.067.694388
GATCGAT2550.066.367048
CTCGATA3600.065.813989
CGTTATA4750.065.556669
CGTGATT4550.065.46279
TCCGATT2700.065.187189
CGCGTAT2200.064.617368
CCGCATA4400.064.617369
AGCGATA5400.062.679989
TAACGAT2600.062.4871258
CGACATT3050.062.1456649
Produced by FastQC (version 0.11.5)