FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790673

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790673
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1541045
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGCCAAGACTTCGGGGGGGGGGGGGGGGGGGGGGGGGGG48010.3115418433595385No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT2800.0108.732979
GACGTAT1800.094.4038248
ACGCGAT2650.088.169628
CGCGATA3800.087.5719769
ACGTATA3050.085.8929
CGACGAA850.083.297498
GATCGAT2100.080.917578
GCGGATT5350.078.081679
GGCGATA4000.077.8831569
GTCGATA3950.077.076549
CGTCTAA2300.076.959648
GCGAATT4300.075.74269
CGGTATA5650.075.188899
CGTTATA3350.073.9731459
ACGGATT5550.071.4407359
GCGTATA4800.070.802879
GTACGAT2000.070.802878
GCGCATA4300.069.156299
CGCTATT3450.067.724499
CGATATA3450.067.724499
Produced by FastQC (version 0.11.5)