FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790675

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790675
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2290682
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGCCAAGACTTCGGGGGGGGGGGGGGGGGGGGGGGGGGG67170.29323144810148244No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT4600.087.9403849
CCGCATA4500.085.244899
GCGCTAT2900.079.365938
GTACGAT3350.079.114668
GATCGAT2500.078.115328
ACCGATA4350.076.960919
GCGAATT5700.074.640269
GGCGATA4650.073.495599
GCGGATT6400.073.015159
CGCGATA5450.072.945169
GACGTAT3200.069.745818
CGGTATA5500.069.745819
ACGTATA5550.067.8607949
ATCGTAT3500.067.753088
CGTGATT6100.067.459079
ACGCGAT3750.066.955988
GCGCATA6400.066.476489
CGAGTTA2100.066.423147
GTCGATA4550.064.380769
AGCGATA7050.064.304669
Produced by FastQC (version 0.11.5)