FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790676

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790676
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2969390
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA222020.7476956546630789No Hit
CGGCGGCTGGTAAGGGAAGAGGGGCTCCTCACTTCCCAGTAGGGGCGGCC92990.31316196255796647No Hit
CTTTCGTGTTCAAACCACGGAGTTCACAACACAGCAGCACACACAGCCGG91700.3088176359454299No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAACTGTCT87900.29602039476121356No Hit
GCCACTTGATTTCTTTTCCTTTCTCTCTCTACCAATATTAAGCCTTAGTG87470.29457228589036805No Hit
AGAAAGAATAAGCATGTTACCTTAAGATCAACCACCATGGACTGAACAAG75160.25311595984360424No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAATTGCCT48710.16404042581136194No Hit
CTGGGCTCATGTGACCTACTGCCTTGGCCTCCCAAAGTGCTGGGATTACA45060.15174833888441736No Hit
TACTAGTCGAAAGCGGCCCCGTGAAGGGGAAGCCGAGGGTGCCGAGACCA43270.14572016474764177No Hit
CAGCACATACACATTTTAGCATACCACAAATTCTTAACCCTTTCATATTC39560.1332260161177885No Hit
CCTGAAGCCACGGGTGTCTTAAGGCTTCTTCTGTCATAAAACGTGCCTTT38980.13127275298967128No Hit
TGGCACACACTTCGAAGTTGAGGGGGGAATTCTGAGGAGGAACTGATGAT34020.11456898554922054No Hit
CTTTCGTGTTCAAACCACGGAGTTCACAACACAGCAGCACACACAGCCAG33270.11204321426286207No Hit
CTGGAAAAGGAGCTCTTGAACCATGTTTGCCCAAGACTGGACAGCGGGCA32840.11059510539201654No Hit
CAAAAGGAGAGCGTATCCAAGAGGCCTAGCAAGAGAATGAGAATCCGCAT32640.10992156638232095No Hit
ACCTGACGGGTATCTTCATGCACGGCAAGATCCCCACGCTGAAGATCTCT32000.10776624155129505No Hit
CAGTCAGCCTGCAGATGTGGATCTAATGATTGACTGCCTTGTTTCTTGCC31720.10682328693772121No Hit
GTAAAAGACTCGGATGATGTACCTATGGTGCTAGTGGGAAACAAGTGTGA31450.10591400927463215No Hit
CCAGGAGTTACAGTTATAGATATAGACTGCGGAGACGTTGTCGTAAGCAA30830.10382603834457582No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATACT2950.0134.651877
TAGTCGA12200.0133.736824
TAGCGGC10500.0126.3277368
CGACGTA3550.0123.8928765
GCTAGCC32650.0116.0221941
GTTATCG603.4833647E-9113.94551145
TCGCGAG2750.0113.5018541
CGCACGT3950.0111.3467561
CGTGAGT3600.0110.78035145
AGTCGAA14800.0110.2425165
TCGTGTT21200.0109.419534
AAACGCG3850.0105.017229
CGTACCC4950.0104.616611
TAATACG3800.0104.5411766
CGTTGCG6300.0101.340945
CTAGTCG16100.0100.01913
TAGCCCT37950.099.8188253
CGCTCGA3200.099.749099
CGTGTTC23400.099.738545
CGGTTTA2350.099.615991
Produced by FastQC (version 0.11.5)