FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790681

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790681
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3619788
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT15400.0116.681769
GCGAATT12850.0112.965979
ATCGATT14950.0110.295619
CGTAATT22150.0109.438029
GCGCATT21450.0104.13959
CGCTATT19600.0100.6666269
CGCAATT21550.096.789439
GTCGATT15700.096.49899
TCCGATT16000.096.45129
GCCGATT18250.096.1435249
ACGGATT22450.095.4202969
CGACATT17200.094.638339
GCGGATT14900.094.586089
CCGAATT19250.093.711479
AACGATT18100.092.268479
CCGCATT22500.092.076419
CGCCATT26350.091.726979
ACGTATT22050.091.718489
CGGAATT24900.091.408529
TACGATT19800.090.752499
Produced by FastQC (version 0.11.5)