FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005790690

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790690
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1507311
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA110400.7324301355194781No Hit
CGGCGGCTGGTAAGGGAAGAGGGGCTCCTCACTTCCCAGTAGGGGCGGCC63020.4180955356923687No Hit
CTTTCGTGTTCAAACCACGGAGTTCACAACACAGCAGCACACACAGCCGG49350.3274042317743319No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAACTGTCT46650.30949153824260556No Hit
GCCACTTGATTTCTTTTCCTTTCTCTCTCTACCAATATTAAGCCTTAGTG36690.24341360210334825No Hit
AGAAAGAATAAGCATGTTACCTTAAGATCAACCACCATGGACTGAACAAG31140.2065930653992441No Hit
TACTAGTCGAAAGCGGCCCCGTGAAGGGGAAGCCGAGGGTGCCGAGACCA25540.16944081214825607No Hit
CTGGGCTCATGTGACCTACTGCCTTGGCCTCCCAAAGTGCTGGGATTACA24170.16035177876363937No Hit
CCTGAAGCCACGGGTGTCTTAAGGCTTCTTCTGTCATAAAACGTGCCTTT20700.13733065040990214No Hit
CTTTCGTGTTCAAACCACGGAGTTCACAACACAGCAGCACACACAGCCAG19150.12704743745650368No Hit
TGCAGATCCCGTACCAGCTTTGGATCTCGGACAGCAACTCCAGCTCAAAG19000.1260522878158522No Hit
CTGACGGCCATGTCTTCCTTTGGCCACAGGACGCTGTGCAGAGCTGCAAG17560.11649885126559816No Hit
GGGATTTCCATTTCTCTTTCGATGCCGACATACTTCAGGGCATCAGCCTG17450.11576907486245375No Hit
TAATAACTCCCAACTCACTGGTCCTCTAAACCTGCAGCAGGGAGATGGTG17420.11557004493432345No Hit
CTGAAAGGCACTGAAATCATTTGGCCTGTATCTTTTATGAGCACCATCTT17410.11550370162494669No Hit
CAAAAGGAGAGCGTATCCAAGAGGCCTAGCAAGAGAATGAGAATCCGCAT17290.1147075819124255No Hit
AAAATTACGTTCTCTTTTCTAGTCGCCGAGATGACATGGAATCATTAGGA17250.11444220867491844No Hit
GGTACAGGCATCCTTCACCTGCTATTGTTGCAAGAACAGTCAGAATTTTA17130.11364608896239728No Hit
TAGCTACTGCCTGCCTTCGAAGAACGATGATTCAGAAGAAGAGGTGAGAG17090.11338071572489022No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAATTGCCT16720.11092601327794993No Hit
ACATATACAAAAGGATAAACGTTAATCTGCCCTGTGCCTTTGCGTTAATT16620.11026258018418228No Hit
AAGAAAGAGCATCAGGAACAAGCCTTGAGTACCCCAACTTGGTCTCGTAG16580.10999720694667522No Hit
CAGTCAGCCTGCAGATGTGGATCTAATGATTGACTGCCTTGTTTCTTGCC16420.108935713996647No Hit
GTAAGGAACTAAAGGGCCGGTAAGTAGGAAGAGGCAGTGCGGCCCCCTTC16220.10760884780911173No Hit
GCATCTCCCTTGCTACGGGAATTCACTAGATGTGTAATGCTTTGTAAATT16060.10654735485908351No Hit
CGCAAACACCTGCTGTTGTACCAGAATATGGGCATCTGGGTGCAGGCAGA16030.10634832493095321No Hit
CATTTGCTTTGCCATCTCCGCATTGTCCACTCACTTGCTCTGCCCTTCTG16000.10614929500282291No Hit
CTGGAAAAGGAGCTCTTGAACCATGTTTGCCCAAGACTGGACAGCGGGCA15910.10555220521843203No Hit
GAAAATAAAACCCCAGAGGCAGAATTCCGAAGTTCAGCTGCATGCTGGTT15780.1046897421965341No Hit
CCCACCCTAATGCCCTAATGTCTGTCTCTCTGTCCTAGGGCTCCCGGGGC15420.10230138305897059No Hit
AGCATCTGTGGTGTCATGCTAATAGCCAGGCACTGATGGCCTGTGTGTTC15320.10163794996520294No Hit
CCCCCATATAGCTTAATCTGATGGGCATCTGGTGGTCCTTCCCTGCAGTG15160.10057645701517472No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)