FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790696

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790696
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1320175
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA91750.694983619595887No Hit
GCCACTTGATTTCTTTTCCTTTCTCTCTCTACCAATATTAAGCCTTAGTG39870.30200541594864316No Hit
CGGCGGCTGGTAAGGGAAGAGGGGCTCCTCACTTCCCAGTAGGGGCGGCC33120.25087583085575776No Hit
CTTTCGTGTTCAAACCACGGAGTTCACAACACAGCAGCACACACAGCCGG32060.24284659230783798No Hit
AGAAAGAATAAGCATGTTACCTTAAGATCAACCACCATGGACTGAACAAG30410.23034824928513264No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAACTGTCT30080.22784858068059158No Hit
TACTAGTCGAAAGCGGCCCCGTGAAGGGGAAGCCGAGGGTGCCGAGACCA23470.17777946105629935No Hit
CAGCTAACATCATACTTAGACTGCAAACTGGCCGGGGATGGTGCCTCACA18240.13816350105099703No Hit
CTGGGCTCATGTGACCTACTGCCTTGGCCTCCCAAAGTGCTGGGATTACA16320.12361997462457627No Hit
CAGCACATACACATTTTAGCATACCACAAATTCTTAACCCTTTCATATTC15510.11748442441343004No Hit
ACAGAGTCTGGCCATGTTACCCAATCTGATCTTGAACTCCTGCTGTCATC14360.10877345806427179No Hit
CCCAGACTCTAACAGATGGTATGCAGTAGGTACTCACAAGCCTTCCCTGA13950.10566780919196317No Hit
GCAAGGTGACAAGCGAGAAAGCCTTTGCGGATGGCAGCGCAAACCTTAGC13660.10347113072130588No Hit
CTTTCGTGTTCAAACCACGGAGTTCACAACACAGCAGCACACACAGCCAG13550.10263790785312553No Hit
CAAAAGGAGAGCGTATCCAAGAGGCCTAGCAAGAGAATGAGAATCCGCAT13360.10119870471717765No Hit
GCTGTTGTTAAATGAGGATTTCCCCAGATTTGCAAATATTTTCTTTCCTA13270.10051697691593918No Hit
CAGTCAGCCTGCAGATGTGGATCTAATGATTGACTGCCTTGTTTCTTGCC13240.10028973431552635No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCGA7150.0122.881414
CGTGAGT1050.0119.73401145
CGCGAGA1600.0114.0536352
CGTGTCG1001.8189894E-12111.75175145
AGTCGAA8100.0110.138165
CGACGTA1600.0109.8252645
GCTAGCC13500.0106.6499251
CGCTCGA1100.0104.421529
CGCACGT1300.0103.992341
TAGCGGC3950.0102.633218
CTAGTCG8700.0100.988753
TGTCGAC4950.099.644059
ACAAGCG3200.099.238839
GAGCGTC2100.096.5240869
TCGTGTT9600.095.74514
GCCCGAT6250.095.173791
CGGGTTA1300.093.5789265
AGCGGTT2700.093.12645145
AAACGCG2050.092.2864469
CGTACCC1700.091.452091
Produced by FastQC (version 0.11.5)