FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790699

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790699
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4208967
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGCCAAGACTTCGGGGGGGGGGGGGGGGGGGGGGGGGGG103700.24637874328784234No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT8350.086.404979
GGCGATA10750.079.494929
GCGAATT9800.078.62399
ACGTTAT6600.077.474118
CGGGATA14800.073.833059
GCGGATT12550.073.116539
CGTTATA10750.072.978949
CGTGATT8900.071.6208349
CGCGATA8200.070.0467459
GACGTAT3600.070.045088
ACGCGAT5200.066.004028
GCGCATA10100.065.1920249
ACGTATA7750.065.075699
ACCGATA10250.064.921389
CGCTATT9500.064.8854149
CCGCATA9000.064.598679
GTACGAT4900.064.327128
GAACGAT6250.063.881128
ACGGGAT10500.063.3741238
GCGCTAT5550.063.1036838
Produced by FastQC (version 0.11.5)