FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790701

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790701
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3094739
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGCCAAGACTTCGGGGGGGGGGGGGGGGGGGGGGGGGGG59440.19206789328599277No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTATT7750.086.00799
GGCGATA8900.083.7539
GCGGATT11900.080.707699
CGCGATA4600.079.4638069
CGCGATT6800.077.997359
GTCGATT7200.076.650569
CCGCATA6950.075.2826849
ACGTATA6350.073.366319
GCGAATT7550.073.097219
GTACGAT3850.072.605248
TCCGATT5600.071.673259
GCGTATA7400.070.704689
GACGTAT3650.070.692568
CGCTATT8650.070.430359
GTCGATA7200.068.686869
AGCGATA10400.068.227419
AGCGATT10500.067.577639
TCGCATA6700.067.394249
GCGCGAT4900.067.286178
CGGCTAT5450.067.071468
Produced by FastQC (version 0.11.5)