FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790707

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790707
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4481536
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGCCAAGACTTCGGGGGGGGGGGGGGGGGGGGGGGGGGG165250.3687351836513196No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA13000.0109.785519
GGCGATA15350.099.5521559
CTCGATA10700.095.659429
CGCGATT13500.095.040669
GCGGATT20100.093.956849
GCGCATA17350.093.892769
CGGTATA16800.093.105139
CGGGATA20600.090.626619
TCGCATA14100.090.485159
GCGCGAT12000.090.1017468
CCGCATA16900.088.715569
GCGCTAT11800.087.963748
GCGCATT18000.087.699039
CGGCATA20100.085.708729
GCGGATA20600.085.028059
GCCGATA15700.084.936679
GCCGATT16050.084.880899
TCGGATA14650.081.675849
CCGCATT16100.081.4833159
TCGCGAT6650.081.29488
Produced by FastQC (version 0.11.5)