FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790711

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790711
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2707819
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGCCAAGACTTCGGGGGGGGGGGGGGGGGGGGGGGGGGG75260.27793585908068447No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT8750.0103.815489
CGCGATA6750.099.6476069
TCGCATA7050.099.34169
ACGCGAT2550.097.906018
CCGCATA8150.096.143459
GGCGATA9550.092.9406059
CGCGAAA7700.092.756739
GCGGATT14750.092.143139
GACGTAT2900.090.8725368
CGTGATT10100.089.939169
CGCGTAT5350.089.442188
CTCGATA6850.089.082229
GATCGAT3550.087.908578
CGGGATA14650.087.56559
TCCGATT9950.087.113589
GTACGAT2800.086.6876148
GCGCATT10950.086.12399
ACGTATA3900.085.3445059
GTCGATA6800.084.638529
GCGCATA11900.084.492849
Produced by FastQC (version 0.11.5)