FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790714

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790714
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5477449
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCACCCTAATGCCCTAATGTCNNNNNNNNNNNNN162370.29643361353067826No Hit
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA141120.25763818156955914No Hit
CGGCGGCTGGTAAGGGAAGAGGGGCTCCTCACTTCCCAGTAGGGGCGGCC136570.2493313949614136No Hit
AGAAAGAATAAGCATGTTACCTTAAGATCAACCACCATGGACTGAACAAG120450.21990163669255522No Hit
TCTCATACCTCCATTCCAAGCTTTCATNNNNNNNN99710.18203729509850297No Hit
CTGGGCTCATGTGACCTACTGCCTTGGCCTCCCAAAGTGCTGGGATTACA85310.15574768473426223No Hit
TGGCACACACTTCGAAGTTGAGGGGGGAATTCTGAGGAGGAACTGATGAT83370.15220589000463536No Hit
TACTAGTCGAAAGCGGCCCCGTGAAGGGGAAGCCGAGGGTGCCGAGACCA83150.15180424317962612No Hit
AAAGAGCATTTGACTAACCTTATTACAGTGACGCCCAATACCCATTAGGA76870.14033905199299893No Hit
CTGTGTTCTTAGGCGGTATCACCNNNNNNNNNNNN74560.136121760330402No Hit
CAAAGCCTGTTTTTCGCAGATAGGGCTACAGGATAAAACCACAGCTATCA69250.12642746650858822No Hit
TATGAAACCATTGCTTTTAGAATAATCATGGGCCAGACTGGGAAGAAATC67140.12257530832327239No Hit
CTGTACTCGATCAGCCCCTTAACACGACTCGTATAAATGCTGCTGAAATA66060.12060358754595432No Hit
CAACTGAGTAAGTGGCAAGAAAATTACCTGCGTGCTGCATCAAAGTTGCT64820.11833975998681139No Hit
ACCTGACGGGTATCTTCATGCACGGCAAGATCCCCACGCTGAAGATCTCT63130.1152543821037859No Hit
GCATAATTTGTTGGACTCTAAGATCAACACCCTGTTATCATTGTGCCAAG61150.1116395606787028No Hit
CTATTGCTGGCACCATCTGACGTGGCAGGCTGGGGGATTTTTATCAAAGA60120.10975912327070503No Hit
CACACTTGCAGAAACAGTATTGGCTGATCGGTTTGAGAGATTGGTGGAAC59470.10857243946954138No Hit
CCAGGAGTTACAGTTATAGATATAGACTGCGGAGACGTTGTCGTAAGCAA59470.10857243946954138No Hit
CTGAGAATAGCAAAACCAAATGTATCAGCCTCAACACAAGCCTCCAGGCA58520.10683805545245606No Hit
CAAAAGGAGAGCGTATCCAAGAGGCCTAGCAAGAGAATGAGAATCCGCAT57970.10583393838993298No Hit
TCATACCTTTCCCCCTGTGAATCGCAAAGCACCCATTCCACGATCGGCTC57670.1052862381740113No Hit
CTGAAAGGCACTGAAATCATTTGGCCTGTATCTTTTATGAGCACCATCTT57350.1047020246103615No Hit
CCAAGAGGGTTCAAATGAAAGCCGAGTTGTAACTATCCATAAGCAAGATG57300.10461074124104121No Hit
CTTTGAGTAGTATGTTGCTACAATCAGCTCCGTAAATAGCACCATTAGAT56870.10382570426488681No Hit
GAAAACCAGCGGAACCTCCTTCAGATGACTGAAAAGTTCTTCCATGCCAT55960.10216434694325771No Hit
TACTTCTCGAAGACGCGCCTCAGCCTCCCAAAGTGCTGGGATTACAGGCG55490.10130628327164708No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCGA34550.0135.18114
TAGCGGC34050.0128.303048
AGTCGAA40100.0117.345925
CGACGTA6200.0116.502535
TGACCGA48150.0115.635259
TTAGGCG16150.0111.5903559
CCGATGT50450.0111.064553
TACTCGA10300.0110.978854
CTAGTCG41000.0110.6649863
CTTCTCG83250.0110.519633
GCCCGAT50600.0110.458121
ACGGGAG63950.0110.092889
GGTGACG64950.0109.159585
TGTCGAC29000.0108.087539
GTGACCG52150.0107.0347448
CGAAGAC89250.0106.7768868
GACGGGA66250.0106.588328
CGATGTG53750.0104.6371154
CGCACGT7400.0102.348591
CGATACT6550.0101.706417
Produced by FastQC (version 0.11.5)