FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005790716

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790716
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4760859
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCGGCTGGTAAGGGAAGAGGGGCTCCTCACTTCCCAGTAGGGGCGGCC236460.49667507481317974No Hit
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA139040.29204813669129875No Hit
CTTTCGTGTTCAAACCACGGAGTTCACAACACAGCAGCACACACAGCCGG97580.2049630119270493No Hit
CAGCTAACATCATACTTAGACTGCAAACTGGCCGGGGATGGTGCCTCACA89860.18874745082767624No Hit
CCCACCCTAATGCCCTAATGTCNNNNNNNNNNNNN89690.18839037240968487No Hit
GCCACTTGATTTCTTTTCCTTTCTCTCTCTACCAATATTAAGCCTTAGTG76300.16026519583965834No Hit
ACAGAGTCTGGCCATGTTACCCAATCTGATCTTGAACTCCTGCTGTCATC74320.15610628250069997No Hit
GGAAGGGATCCTTGTAATATAACCCACACCATCCCCACAGTGTGAACGTG63940.13430349439040307No Hit
AGGAACTGTTACACATGTAGTTGTAGTGGATGGTGGTACAGTCAGAGCCA62450.1311738070797728No Hit
TACTAGTCGAAAGCGGCCCCGTGAAGGGGAAGCCGAGGGTGCCGAGACCA60910.1279390967050274No Hit
CAGTCTAGCCTTTGAATTGATGCGCTTGACCTCTGTGCTCTTCTGGCTGC60810.12772905057679718No Hit
CGAAGTGCGGAAGATGTGCAACTACACCAAGATCCTGTCCACCAAGAAGA59030.12399022949429925No Hit
GGTAGGGTAGGGAAGTTCACAGAGAAGTCTCGAGAGTTTAGCTCTGTCCA58750.12340210033525462No Hit
TAGCTACTGCCTGCCTTCGAAGAACGATGATTCAGAAGAAGAGGTGAGAG58320.12249890198386466No Hit
CTGGGCTCATGTGACCTACTGCCTTGGCCTCCCAAAGTGCTGGGATTACA58200.12224684662998841No Hit
GATGATCTCATCGCCATGCTTGTTGACAGTGCGAGTCTGTGTTGCCGTCA57870.12155369440682867No Hit
CCTCCTCCTCCTCTTTGCGTTTCTTGTCTTCTTCCTCCTCCTTAAGCCTC57040.11981031154251785No Hit
GCCTCAGCAAAGCTTCCCGTGTCCCCCAGGTTCGCTGACCACCTGCACAA55550.11668062423188758No Hit
CTGACGGCCATGTCTTCCTTTGGCCACAGGACGCTGTGCAGAGCTGCAAG55060.11565139820355949No Hit
CAGTGGGTAGAAATGGTTGTGTGATGTCTTGCCTTCCAGGTCAATGTGGA54680.11485322291628465No Hit
CATCCGTGGATAATGTGCACCATAACCCTCCCACCATTGAACTGTTGCAC53900.11321486311608893No Hit
GGCAAGATTGAAGGTAGGAGATAAGAGGTGGGGCAGAAGAAGGTCAGCAT53720.11283678008527454No Hit
CCTGGGATCAAGAACCTTCCCCCACCCCAGGCTACTGCCAAACCCCACAA52670.11063129573885722No Hit
GCCAGCGGATGCAGCGTATCTGCAGGGTGTAGGCCGTGGCTGGGAGGAGC52350.1099591481285205No Hit
GGAGATGGAGAGAGGAGAGCAGGACGGGAGGAGCTGGCAGTGGAAGGCCC52080.10939202358229891No Hit
GAAAATGGAAGTGAGTGCCGGTTGGTTAATTCTCTTCCTGTCCAGGGATC52070.10937101896947589No Hit
GCCAGCCGTCGTACCTGTAGTTGTGGAACCAGTTGATGACGGTGCTGGTT52010.10924499129253776No Hit
TAGCGCTGGAAGAGGTCGGTGAACTGCAGCGACACCAGCTCGGCGCGCAG51730.10865686213349314No Hit
CCCAGCCGATACTGCACGTGACTATCCACCACCAGCTTAGTCAAACGCAG51680.10855183906937803No Hit
CAAACAGACGCTGGGCTATCGAAGCCCCGACGCCGGGCCACCGCACGCTT51410.10798471452315643No Hit
CCCCACCCACGTGTCTTTCAGCCCGGCCGCACCACCTGGGTCTCCGCCAT51310.1077746683949262No Hit
GGCAGGCCTGATTCAATGACTCTGTGGGGCTGGGTCTTAGGTACCCCATC51040.10720754384870462No Hit
CGACCTTGATCCATTCTTCGTCAGTCAGAGTGGGCCAGATGTGGTGTGGT50530.10613630859473049No Hit
GCGAGATGGGCATTTTAAGAAACGCTCTCGGCCAGATCTCCGAACCAGAG50430.10592626246650028No Hit
CATCTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATC50420.10590525785367726No Hit
CAGCCTCCTGCATCATCAAGCAGAACTGCAGCCATCTGGACCCGGAGCCA49800.10460297185864989No Hit
CAGTGAATGCCGTCGGTGAAGTCCGTCGACCTGTTGTCAGGCATGGCCAC49210.10336369970209158No Hit
GCCTGACCTTAGTCTGCCATCTCTTCCTCCGCAGCCTACATGAAGCGGCG49170.10327968125079948No Hit
GGTAAAAGACAGCTATTTTCAGGCACGGTTTCTCGTGTGCTTTAATTACA49070.10306963512256927No Hit
CCCCCGAGCAGTACGAGGTCTACATGTACCAGGAGGTGGACACCATCGAG48910.10273356131740091No Hit
TTGTCCCATGCCTATTGTCAGATAAGCACTGCCTCCCTTTGTCCCTGGCC48560.10199839986859513No Hit
ACCTGACGGGTATCTTCATGCACGGCAAGATCCCCACGCTGAAGATCTCT47990.10080113693768289No Hit
CTCGCTGCCACTTCAAGTCACGAAATGACAGCATTATTCACATCCTTGTG47780.10036004006839941No Hit
ATGGAGCCAGAGGAGGAGGATGAACTAGGCTCAGGGCGGGATGTGGATTC47730.10025501700428431No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)