FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790719

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790719
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4636636
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGCCAAGACTTCGGGGGGGGGGGGGGGGGGGGGGGGGGG202460.4366527801621693No Hit
GGGGGGGGGGGGGCCAAGACTTGGGGGGGGGGGGGGGGGGGGGGGGGGGG59860.12910221979900946No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA10200.097.641249
CCGCCTA14750.085.725137
GCGAATT12800.084.429819
GCGGATT17800.081.745079
GATACCG13400.081.716833
CGCGATT11800.081.408789
ACGGATT19200.080.567019
GCGCATT16650.080.179249
TCGCGAT4950.079.913588
ATACCGC13900.079.792144
GCGTATT14250.078.812779
GCGCATA16350.078.626339
TTAGGGC41600.078.6254044
ACGGGAT14800.077.797148
CCGCATA11000.076.4132469
CGCCTAA17400.075.9154368
GTACGAT7150.075.083648
GGCGATA14600.074.988249
GACGTAT6900.074.732858
ACGTATT13550.074.5437249
Produced by FastQC (version 0.11.5)