FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790725

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790725
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3639352
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGCCAAGACTTCGGGGGGGGGGGGGGGGGGGGGGGGGGG108500.29812999676865554No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTAT3600.082.113538
GCGCATA11950.081.864759
GCGCATT12500.081.6409459
GCGGATT12950.080.434439
GGCGATA9150.079.9948969
GATCGAT4400.079.980718
CGTGATT10850.079.785779
CGCGATT7800.079.403229
CGCGATA7800.079.403229
CGGTATA11100.078.622859
TCGCATA8100.078.200159
GCGCTAT5650.077.2344748
ACGCGAT4500.076.6393058
GCGTATT12650.076.2219549
GTACGAT4200.075.4103858
ACCGATA8200.073.81339
ACGGATT14950.073.440139
CGGGATA14000.072.893719
GCGAATT10900.071.671519
CGACATT7950.070.822779
Produced by FastQC (version 0.11.5)