FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790726

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790726
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2058287
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA110230.5355424194973781No Hit
CGGCGGCTGGTAAGGGAAGAGGGGCTCCTCACTTCCCAGTAGGGGCGGCC97040.471460005334533No Hit
CTTTCGTGTTCAAACCACGGAGTTCACAACACAGCAGCACACACAGCCGG57470.27921276284599766No Hit
AGAAAGAATAAGCATGTTACCTTAAGATCAACCACCATGGACTGAACAAG52580.25545514303884737No Hit
GCCACTTGATTTCTTTTCCTTTCTCTCTCTACCAATATTAAGCCTTAGTG47890.23266920502340052No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAACTGTCT45670.2218835371354918No Hit
TACTAGTCGAAAGCGGCCCCGTGAAGGGGAAGCCGAGGGTGCCGAGACCA40060.1946278628782089No Hit
CCTGAAGCCACGGGTGTCTTAAGGCTTCTTCTGTCATAAAACGTGCCTTT35350.17174475668359174No Hit
CTGGGCTCATGTGACCTACTGCCTTGGCCTCCCAAAGTGCTGGGATTACA28980.14079669161783562No Hit
CAGTCAGCCTGCAGATGTGGATCTAATGATTGACTGCCTTGTTTCTTGCC26900.13069120098411932No Hit
ATTGATTAGTGGCATCTGTATATTTATTTTAAACACTGCTAATAATCTTT23370.11354101736055275No Hit
GCCTCAGCAAAGCTTCCCGTGTCCCCCAGGTTCGCTGACCACCTGCACAA23350.11344384918138238No Hit
GGTACAGGCATCCTTCACCTGCTATTGTTGCAAGAACAGTCAGAATTTTA23300.1132009287334565No Hit
AAGATACTTATAGCTTCTTGTCTCCAGGTCTGTAGGTTTTTCTTTAAATT22900.11125756515004953No Hit
TGGCACACACTTCGAAGTTGAGGGGGGAATTCTGAGGAGGAACTGATGAT22890.11120898106046437No Hit
GCATAATTTGTTGGACTCTAAGATCAACACCCTGTTATCATTGTGCCAAG22540.10950853792498325No Hit
CTTTCGTGTTCAAACCACGGAGTTCACAACACAGCAGCACACACAGCCAG22240.10805101523742801No Hit
CTGACGGCCATGTCTTCCTTTGGCCACAGGACGCTGTGCGGAGCTGCAAG21580.1048444653248065No Hit
CACTTCCTCCTGAATGTACCCCCAACATAGATGGACCAAATGCTAAATCT21440.10416428807061406No Hit
ATACTGCCCAATTTTCTCCTGATTGTTCCAGAGTAGACGATGTAAAGCAA21150.102755349472644No Hit
GAAAATAAAACCCCAGAGGCAGAATTCCGAAGTTCAGCTGCATGCTGGTT20690.10052048135172599No Hit
CTTGTAGATTTTACTTTCCTCGTTGGTATTGCAGTGGCAGCAACAGAGCC20590.10003464045587422No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCGA9750.0135.481054
TCGCGAG1200.0131.650621
CGTGAGT2850.0129.38948145
TAGCGGC9650.0119.748198
CGACGTA2100.0119.65045
AGTCGAA11100.0119.003635
CGATACT1750.0118.940667
TGTCGAC9900.0118.899239
AAACGCG2550.0118.216899
GGACGTA1800.0115.662045
GTGTCGA10400.0113.182928
GCTAGCC16350.0111.996361
CGCGAGA1500.0110.078352
TGACCGA13500.0108.45939
CTAGTCG12300.0107.977183
CGCAATC1800.0107.685353
CGCTCGA1750.0106.636459
CATACCG2450.0105.4646159
CGGTTTA1850.0104.802941
TAATACG1800.0103.697016
Produced by FastQC (version 0.11.5)