FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790727

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790727
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2058287
Sequences flagged as poor quality0
Sequence length37-151
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGCCAAGACTTCGGGGGGGGGGGGGGGGGGGGGGGGGGG54590.26522054504546744No Hit
GGGGGGGGGGGGGCCAAGACTTGGGGGGGGGGGGGGGGGGGGGGGGGGGG22670.11014013108959053No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21170.10285251765181434No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTAT2750.0102.23698
CGCGATA3800.092.958329
ACGTATT5500.090.440349
CGCGATT5150.089.587729
GCGAATT6200.089.531289
GCGGATT8150.086.685059
CGGTATA6950.085.055979
ACGCATA4850.083.2380759
GTCGATT4950.080.1001369
ACCGATA5300.078.8910759
GCGCATT7150.078.643779
CGTGATT7900.078.477859
ACGGATT7650.078.215429
GCGCTAT4150.078.170018
GCGTATT7400.075.9868859
GCGCGAT4000.075.6946268
ACGCGAT2200.075.366948
TCCGATT5100.074.917189
GATCGAT3400.074.210428
GCCGATT6050.073.877489
Produced by FastQC (version 0.11.5)