FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790730

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790730
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2985799
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCGGCTGGTAAGGGAAGAGGGGCTCCTCACTTCCCAGTAGGGGCGGCC188050.6298146660240693No Hit
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA122790.4112467048183753No Hit
CTTTCGTGTTCAAACCACGGAGTTCACAACACAGCAGCACACACAGCCGG80140.26840386777542624No Hit
CTGGGCTCATGTGACCTACTGCCTTGGCCTCCCAAAGTGCTGGGATTACA67950.2275772749605717No Hit
GCCACTTGATTTCTTTTCCTTTCTCTCTCTACCAATATTAAGCCTTAGTG63060.21119974921285728No Hit
CAGCTAACATCATACTTAGACTGCAAACTGGCCGGGGATGGTGCCTCACA54230.1816264256234261No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAACTGTCT53340.17864564895359666No Hit
AGAAAGAATAAGCATGTTACCTTAAGATCAACCACCATGGACTGAACAAG48000.16076098893462018No Hit
CCTGAAGCCACGGGTGTCTTAAGGCTTCTTCTGTCATAAAACGTGCCTTT47000.15741180166514893No Hit
GTAAAAGACTCGGATGATGTACCTATGGTGCTAGTGGGAAACAAGTGTGA46180.1546654681041825No Hit
TACTAGTCGAAAGCGGCCCCGTGAAGGGGAAGCCGAGGGTGCCGAGACCA45170.15128278896201652No Hit
CGTTTGCATCACTAACACTACTATCAGGGTAATCGTTAAATAGATTCATG41080.13758461302987912No Hit
GGGTCCTTTCCTGACTGTCCCTCCAGACACTAGCACATGCGGAGAATTAA41010.13735016992101612No Hit
CGTCTCCTCGCCGCGATCCTTTCTGGCGAGTCCCCGTGCGGAGTCGGAGA40190.1346038363600497No Hit
CTTTCGTGTTCAAACCACGGAGTTCACAACACAGCAGCACACACAGCCAG36910.12361850211618397No Hit
GAAACTGGCTGAGCACATAGAGCATGAACAACAGAAACTACCTGCTGCCA34880.11681965195915733No Hit
CACACACACACACACAGTTTATTGCATTGTTAGATTTTATACATAAAATT32840.10998730992943598No Hit
CCCAGCCTAGTTCTATTTTCTTCCTTTTGCAAGGGACACTGTAATCACTA32690.1094849318390153No Hit
CAGTCTAGCCTTTGAATTGATGCGCTTGACCTCTGTGCTCTTCTGGCTGC32340.10831271629470035No Hit
GAATTTGGGACTCTGCCATATTCTTTCCGTCTATTTAAAAGGATTGGATT31920.10690605764152243No Hit
AGGAACTGTTACACATGTAGTTGTAGTGGATGGTGGTACAGTCAGAGCCA31900.10683907389613298No Hit
CCAATCTGCTCACCTGCTTGAGTTGTAGTACTGTACCCAATGTTTCCACC31790.10647066329649114No Hit
CCAACTAGATCAATGAGACCCACGTGTAAATGTAGCCACTTACTTTTGAT31730.10626971206032289No Hit
CGACCTTGATCCATTCTTCGTCAGTCAGAGTGGGCCAGATGTGGTGTGGT31060.10402575658977715No Hit
GACCAGAAATTTCCCCAATGATATATACAGCCTTCCCCAATAACCAAGGC30910.10352337849935644No Hit
TACTTCTCGAAGACGCGCCTCAGCCTCCCAAAGTGCTGGGATTACAGGCG29940.10027466684796933No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCGA12300.0132.150074
TAGCGGC27250.0131.622658
CGACGTA3500.0126.844115
AAACGCG3350.0123.9488759
CGATACT2700.0121.9701167
TAATACG3700.0119.987676
TGACCGA21400.0116.0847249
CGCACGT6000.0115.804981
GTAGCGG31600.0113.277157
CTAGTCG14450.0111.0013
AGTCGAA15050.0108.0030445
CCGATGT23300.0105.718063
CGCTCGA3000.0105.000379
CGGTTTA2950.0104.412661
GGTAGCG34500.0103.983716
TCGTGTT24050.0103.3144454
CATGTGA74100.0102.217888
CTTCTCG44950.0102.111593
CGATGTG24650.0100.218724
GCCCGAT24750.099.850591
Produced by FastQC (version 0.11.5)