FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790731

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790731
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2985799
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGCCAAGACTTCGGGGGGGGGGGGGGGGGGGGGGGGGGG76320.2556099724060461No Hit
GGGGGGGGGGGGGCCAAGACTTGGGGGGGGGGGGGGGGGGGGGGGGGGGG30160.101011488047253No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT8700.093.5082559
CGCGATA7600.088.0967949
ACGGATT11600.087.508839
GCGGATT13250.086.391699
CGGTATA9500.086.391699
GTACGAT4650.085.154538
TCGCGAT4300.082.048
GCGCATT12350.081.028659
ACGGATA10500.080.906519
GCGAATT8750.080.632259
GCGTATT9900.079.265119
ACGCATA5350.078.048579
GCGCATA12150.076.4370969
CGCTATT9400.075.8224959
TCGCATT7600.075.782199
CCGCATA7750.075.244389
ACGCGAT4350.074.476858
GCGGATA12950.073.9388359
CGGGATA13150.073.909249
ACGGGAT10750.073.668588
Produced by FastQC (version 0.11.5)