FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790734

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790734
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1863583
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCGGCTGGTAAGGGAAGAGGGGCTCCTCACTTCCCAGTAGGGGCGGCC180750.9699058212057096No Hit
CGTCTCCTCGCCGCGATCCTTTCTGGCGAGTCCCCGTGCGGAGTCGGAGA103860.5573135191724758No Hit
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA99670.5348299485453559No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAACTGTCT79070.4242901979681077No Hit
CTTTCGTGTTCAAACCACGGAGTTCACAACACAGCAGCACACACAGCCGG60410.3241605015714352No Hit
GCCACTTGATTTCTTTTCCTTTCTCTCTCTACCAATATTAAGCCTTAGTG42070.22574792751382686No Hit
CTGGGCTCATGTGACCTACTGCCTTGGCCTCCCAAAGTGCTGGGATTACA40720.21850381764590038No Hit
AGAAAGAATAAGCATGTTACCTTAAGATCAACCACCATGGACTGAACAAG36590.19634220745735498No Hit
CAGCTAACATCATACTTAGACTGCAAACTGGCCGGGGATGGTGCCTCACA34830.18689803459250273No Hit
AGGCCGCCCTAGAGCATTCTAAGCCGCGGGACCCGGGCACCCCCCGACAT34170.18335646976818312No Hit
TACTAGTCGAAAGCGGCCCCGTGAAGGGGAAGCCGAGGGTGCCGAGACCA33030.17723922143526744No Hit
CGTTTGCATCACTAACACTACTATCAGGGTAATCGTTAAATAGATTCATG31270.16779504857041516No Hit
TCATGGTGACGGGAGCAGCCTCTACCAGCCTGTCCCAGGTGGTGAGCCCT30630.16436080389228705No Hit
TTTTTAAGATAACTTTACTCACTTTTTCTATACTTGCTTGCATTTTTGCT29740.15958505738676518No Hit
GCCCGATGTGACCGAGCCCAGTCCGTCCTGTCTCAAATATGTACGTGCAA29310.15727767424364786No Hit
CACACACACACACACAGTTTATTGCATTGTTAGATTTTATACATAAAATT28800.15454101051576452No Hit
CGGCGGCTGGTAAGGGAAGAGGGGCTCCTCACTTCCCAGTAGGGGCGGCT27660.14842376218284883No Hit
CTTTCGTGTTCAAACCACGGAGTTCACAACACAGCAGCACACACAGCCAG27490.14751154094022106No Hit
GCATGCCACGGTCCCAACGGGCCCCCGGGGCTCTGCTTTCGAGAACCCGG27410.14708226035545507No Hit
CAGCCGCTCACTCCGTCTCAATATGTCTCAAGATGGCGGCCAATGTGGGA26770.14364801567732693No Hit
CACTTGCTGCACCTTAGAGAACGATGGGAGCAGCAGGTGTCGGCAGCAGA26380.14155527282659264No Hit
TGTGCACAAAGGAGATACTAGATAAATATTTGAGCAAACTCAATACCTGC25980.13940886990276258No Hit
CCTGAAGCCACGGGTGTCTTAAGGCTTCTTCTGTCATAAAACGTGCCTTT24900.1336135820084214No Hit
GCCATGTGCTTTGAGGCAGACTGAGTAAAAAAAACCACTATTCACATTTA24810.13313064135055966No Hit
CGACCTTGATCCATTCTTCGTCAGTCAGAGTGGGCCAGATGTGGTGTGGT24250.13012567725719756No Hit
AGGAACTGTTACACATGTAGTTGTAGTGGATGGTGGTACAGTCAGAGCCA24200.1298573768917188No Hit
AACCTGAGAACTTCCTGATCTCTCTCGCTGTGAGACATGTCTGAGACTCC24090.12926711608766553No Hit
CAAAAAGACCACTGCTGGGTTTCCTTAAGTCTGTCTTTCCCCAGAAGGAG23930.12840855491813352No Hit
CAGTCTAGCCTTTGAATTGATGCGCTTGACCTCTGTGCTCTTCTGGCTGC23870.128086594479559No Hit
GGGTTGCCCTGCGAGTCGAGGTGATTGAAGAAGACGTCCACCTCGTCTGG23830.12787195418717598No Hit
GTAAAAGACTCGGATGATGTACCTATGGTGCTAGTGGGAAACAAGTGTGA23510.12615483184811194No Hit
CCTGGCCTGAAAATACATGGTCCTGAGCCTCAACTCACCTGTGCAAGTTA23150.12422306921666489No Hit
CCAATCTGCTCACCTGCTTGAGTTGTAGTACTGTACCCAATGTTTCCACC23010.12347182819332436No Hit
GGCAGCAGTGTCGACGGCAGCGGCGGCGGCGGGTGGGAAATGGCGGAGTA22460.12052052417305802No Hit
CCTCCTCCTCCTCTTTGCGTTTCTTGTCTTCTTCCTCCTCCTTAAGCCTC22210.11917902234566424No Hit
GGTGCACTTACTCTGTGTGTTTAGATCAGTCAGTTTCATCTCTCTAGGGG22200.11912536227256848No Hit
TGGCACACACTTCGAAGTTGAGGGGGGAATTCTGAGGAGGAACTGATGAT21620.11601307803301489No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAATTGCCT21240.11397399525537634No Hit
CTCTAGTGAAACGCGGTCCTTGACACTAGCACGGCAGACCAGATGGAGTG21190.1137056948898976No Hit
TTCGGGCTTCATTAATGCCCTCTTGTCTCCCCTCCCCCAGGCGAGTATAG21130.11338373445132306No Hit
CAGCACATACACATTTTAGCATACCACAAATTCTTAACCCTTTCATATTC21100.1132227542320358No Hit
CTTTTCTCCAAAATTCCGCAGCATCTGCTTTAGTAATCCGAAATGTGTCT21030.11284713372036555No Hit
CCTCTGTCCCCTTCTCTCATCTCTTAGCTCCCCTCCAACCTGGGGGGCAG20790.11155929196606752No Hit
CATGCATGTGTATGTTTAGTGCAGCACTATATACAATAGCAAACACATGG20690.11102269123511001No Hit
CTGCTCTTCCTACTCCTTTTGGGTGGAGCTTATCAGGTTCTCCATTGGCA20590.11048609050415248No Hit
CGAGCCCCTCTTCAGCTTGGTCAGTGGCCTAGTGAGCATGACCAACCCAC20370.10930556889604595No Hit
CCTTCAGGTGAATGGGAAAGGACCGCTCCTGCTGGAGGGGCCCCAGCAGC20330.10909092860366294No Hit
ACACAGACTGGAACTTACTTTAATAGAAACTTTGGTGTCTTTGTGAGCTA20040.10753478648388615No Hit
CAATCTCCTAACAAACAAAACTTAAAAGGCACCATGCACTTAATTGGCCG20020.10742746633769465No Hit
TAGCTACTGCCTGCCTTCGAAGAACGATGATTCAGAAGAAGAGGTGAGAG19980.10721282604531164No Hit
CTTGCCAAGTTGCTGTAGAAATTTCAAATGTCTCTCTTCAAACTGTGTAG19930.10694452567983287No Hit
ACAGAGTCTGGCCATGTTACCCAATCTGATCTTGAACTCCTGCTGTCATC19600.10517374326767309No Hit
TGGAGCTGACCAGTGACAATGACCGCCTGCGCAAGCGGGTGGAACAGCTG19360.10388590151337504No Hit
GCCTGACCTTAGTCTGCCATCTCTTCCTCCGCAGCCTACATGAAGCGGCG19340.10377858136718354No Hit
TTGTCCCTCACATGTGCACTCTTCCAAATTTCAGAAAAGCAAGAGGAAAG19290.10351028100170478No Hit
CTGGGAGTGAGCGTCTTCTCCGACCAGGGCGGGAGGAAGTACATGGAGGA19020.10206145902811947No Hit
GAAACTGGCTGAGCACATAGAGCATGAACAACAGAAACTACCTGCTGCCA19010.10200779895502374No Hit
GCCTGCAGTTTGCCTCATTCATCGTGCAGTATTATGGCCTGGTGATGGAT18990.10190047880883223No Hit
GCACTAAACATACACATGCATGTATTTTTATAACAGAACGTTTTATATTC18930.10157851837025772No Hit
TAGATTAAACACTCAACAAAGTAAACATGTTTCTGTCCTCTGTCTCATGT18900.10141753815097046No Hit
AAACAGTGGATCATGGGATATGCCGGAGGGACCAGTGGTAACCAGCAGCG18770.1007199572007257No Hit
AGATCTCTGACCCGTTCGGATCCTTTCCTCACTCGCCCACCATGGACAAC18680.10023701654286393No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCGA8000.0137.71674
CGACGTA1350.0132.48365
TAATACG2000.0132.351126
TAGCGGC22650.0130.430598
CGTGAGT2200.0129.87683145
CGATACT1750.0126.713027
AAACGCG2950.0126.089499
TGACCGA16550.0124.045639
TTAGGCG3500.0122.62559
AGTCGAA8950.0122.299385
TGTCGAC6450.0121.991789
GTAGCGG24750.0119.652767
CGCACGT3150.0118.125031
CTAGTCG9550.0115.364783
GCTAGCC13750.0114.490411
TAAGCGT1500.0114.291595145
CCGATGT18300.0114.153083
GCTGGTA54350.0111.359356
CGATGTG18700.0111.3287354
GCCCGAT19000.0109.217831
Produced by FastQC (version 0.11.5)