FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005790736

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790736
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4292303
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA290100.6758609538981754No Hit
CGGCGGCTGGTAAGGGAAGAGGGGCTCCTCACTTCCCAGTAGGGGCGGCC195410.4552567700835659No Hit
GCCACTTGATTTCTTTTCCTTTCTCTCTCTACCAATATTAAGCCTTAGTG152760.3558928621767848No Hit
CTTTCGTGTTCAAACCACGGAGTTCACAACACAGCAGCACACACAGCCGG143950.3353677501332036No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAACTGTCT121670.28346088335329545No Hit
AGAAAGAATAAGCATGTTACCTTAAGATCAACCACCATGGACTGAACAAG88620.20646259129423064No Hit
CTGGGCTCATGTGACCTACTGCCTTGGCCTCCCAAAGTGCTGGGATTACA74370.1732636302702768No Hit
CTTTCGTGTTCAAACCACGGAGTTCACAACACAGCAGCACACACAGCCAG68480.15954139304704257No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAATTGCCT66320.15450912948130643No Hit
CCTGAAGCCACGGGTGTCTTAAGGCTTCTTCTGTCATAAAACGTGCCTTT64040.1491972957174738No Hit
TACTAGTCGAAAGCGGCCCCGTGAAGGGGAAGCCGAGGGTGCCGAGACCA62990.14675105648412984No Hit
CTGTACTCGATCAGCCCCTTAACACGACTCGTATAAATGCTGCTGAAATA58080.13531197587868332No Hit
TGGCACACACTTCGAAGTTGAGGGGGGAATTCTGAGGAGGAACTGATGAT57210.13328509194248403No Hit
CAGCACATACACATTTTAGCATACCACAAATTCTTAACCCTTTCATATTC56920.13260946396375092No Hit
GTCTGAGGAAAATGAATCCACAGCTCTACCCCAGGTTCTAGCCCAGGTAT52350.12196249891957768No Hit
GGAGAAACAAATGCTGCTCCGATCATCTGCTCTGCTTCTTCTGTTCCTCA51190.1192599870046453No Hit
GGCTTATGTCTAAGTTTTTCAAGCACAGGGTAGAATATTGGGCTGAGTAA50600.11788543353067106No Hit
TAGATTAAACACTCAACAAAGTAAACATGTTTCTGTCCTCTGTCTCATGT50340.11727969810146209No Hit
AAGATACTTATAGCTTCTTGTCTCCAGGTCTGTAGGTTTTTCTTTAAATT49540.11541589678081905No Hit
GGGATTTCCATTTCTCTTTCGATGCCGACATACTTCAGGGCATCAGCCTG49020.1142044259224011No Hit
AAAATTACGTTCTCTTTTCTAGTCGCCGAGATGACATGGAATCATTAGGA46960.10940513752174533No Hit
CTTTGAGTAGTATGTTGCTACAATCAGCTCCGTAAATAGCACCATTAGAT46310.10789079894872287No Hit
CTGAAAGGCACTGAAATCATTTGGCCTGTATCTTTTATGAGCACCATCTT46060.10730836103602193No Hit
CTGAGAATAGCAAAACCAAATGTATCAGCCTCAACACAAGCCTCCAGGCA44960.10474563422013777No Hit
CCCAGACTCTAACAGATGGTATGCAGTAGGTACTCACAAGCCTTCCCTGA44320.10325459316362336No Hit
ACCTGACGGGTATCTTCATGCACGGCAAGATCCCCACGCTGAAGATCTCT44220.10302161799854298No Hit
CATTTCTCTCATTAGCCCGGTTTTCAGATACTCAATACCAAAGAATTGAA43760.10194993223917323No Hit
CTTGGTTCTTCAGTGTCTGTTCATATTCTCGGATTTTCTCTTTAAGTGCT43520.10139079184298032No Hit
GCTTTTCCCTACAAAATAGAATACATCCCTGAGAAATGGCAGAGAAACAA43480.10129760177694817No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)