FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790744

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790744
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5834083
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA277860.4762702210441641No Hit
GCCACTTGATTTCTTTTCCTTTCTCTCTCTACCAATATTAAGCCTTAGTG150440.2578640036489025No Hit
CTTTCGTGTTCAAACCACGGAGTTCACAACACAGCAGCACACACAGCCGG122770.21043581313464343No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAACTGTCT105050.18006257367267486No Hit
AGAAAGAATAAGCATGTTACCTTAAGATCAACCACCATGGACTGAACAAG101020.17315488998013912No Hit
CCTGAAGCCACGGGTGTCTTAAGGCTTCTTCTGTCATAAAACGTGCCTTT73280.1256067148856127No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAATTGCCT73230.12552101161399315No Hit
CAGCACATACACATTTTAGCATACCACAAATTCTTAACCCTTTCATATTC67420.11556229145180143No Hit
CGGCGGCTGGTAAGGGAAGAGGGGCTCCTCACTTCCCAGTAGGGGCGGCC60480.10366667735100786No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGCC39750.093.8981861
CGACGTA6000.092.572565
TCGTGTT36300.089.479054
TAGTCGA12050.088.6812744
CGTGTTC37600.086.70655
CGCACGT9650.084.491361
CACGGGT24300.081.956969
CGTGTCG3250.081.412994145
CGATACT6150.079.485537
CGTCGGA15000.078.887925
GGACGTA4550.075.632625
CGTGAGT4250.075.597786145
CACGTCG10900.075.330233
TAGCCCT50700.074.545813
TAGCGGC16850.074.4974758
AAACGCG7050.074.474629
CTAGTCG14450.074.371933
CGGTTTA5650.072.68861
GACGTAA4900.070.230296
CGCAATC5750.068.249963
Produced by FastQC (version 0.11.5)