FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790747

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790747
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3929440
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGCCAAGACTTCGGGGGGGGGGGGGGGGGGGGGGGGGGG147660.3757787369192557No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT8300.083.337339
CGTGATT8950.080.965149
GCGCATA10750.079.0514149
GCGAATT8350.078.104699
ACGTTAT6450.077.6225368
CCGCATA9400.076.388349
ATCGATT10000.075.098859
ACGTATA8700.074.962559
GCGCATT11550.071.864929
GCGGATT12650.070.82349
CGTTATA9500.070.036789
GACGTAT4650.069.418798
TCGCATA9050.067.695969
CGCGATA9150.066.236169
CTCGATA10250.065.554839
CGATATA10050.065.548449
CGTTATT14150.064.7122969
ACCGATA7250.064.513229
TACATTA41550.063.83239145
TGCGATA9200.063.0129
Produced by FastQC (version 0.11.5)