FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790750

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790750
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4143518
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA247890.5982597396704926No Hit
GCCACTTGATTTCTTTTCCTTTCTCTCTCTACCAATATTAAGCCTTAGTG161060.38870351232937805No Hit
AGATGTTTGGAGCTTTGGAGTGGTTCTGTATCNNN156660.3780845165871127No Hit
CTTTCGTGTTCAAACCACGGAGTTCACAACACAGCAGCACACACAGCCGG119590.2886194774585268No Hit
TCCACGTTGCGCTGCTTGGCGCGGATCGCACGCAGGAACCATGTGATGTC116460.2810655100327789No Hit
CCCACCCTAATGCCCTAATGTCNNNNNNNNNNNNN95060.22941857619539724No Hit
GCCGCCAAGGAGCGGGAGATCCATCGCAAGCTGCAAGCGTTCTGCGCAAG93710.22616047522902036No Hit
CTGTGTTCTTAGGCGGTATCACCNNNNNNNNNNNN92050.22215421774443841No Hit
CGGCGGCTGGTAAGGGAAGAGGGGCTCCTCACTTCCCAGTAGGGGCGGCC76220.18394996715351544No Hit
CGTCTCCTCGCCGCGATCCTGGAGCGTGAGGTCGCCGCCAGGAACGCCTC75800.18293633574175375No Hit
CAGCACATACACATTTTAGCATACCACAAATTCTTAACCCTTTCATATTC75490.18218817922354869No Hit
GGGCATCGGCATCGGCATGTCGGCGCGGTTCGCCGAGGTGTCGATGATGC69350.16736985334684198No Hit
TCTCATACCTCCATTCCAAGCTTTCATNNNNNNNN68520.165366724604551No Hit
CGGCGGCTGGTAAGGGAAGAGGGCATCTTCTGCGGCGTTTCGTCTGGCGG66340.16010549489588316No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAATTGCCT64020.15450638804996142No Hit
CCTGAAGCCACGGGTGTCTTAAGGCTTCTTCTGTCATAAAACGTGCCTTT63490.15322728174464306No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAACTGTCT59290.1430909676270261No Hit
AGAAAGAATAAGCATGTTACCTTAAGATCAACCACCATGGACTGAACAAG57020.1376125311872665No Hit
CGTCTCCTCGCCGCGATCCTCGAGCCGTCCGCCCTCGGCGTCATCACCAT53370.12880359153743268No Hit
TAGATTAAACACTCAACAAAGTAAACATGTTTCTGTCCTCTGTCTCATGT48050.11596426032178453No Hit
GAACAGGTCGGCCAGGAACTCGTCGGTGGTCTTCTGGATCACCTGATGCG46190.11147532121255416No Hit
CTTTCGTGTTCAAACCACGGAGTTCACAACACAGCAGCACACACAGCCAG44310.10693811394085896No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGGC54950.099.265178
TTAGGCG15650.094.301449
GGTAGCG57950.094.048326
GTAGCGG58350.093.278747
GCTAGCC36100.092.7738341
CGTTGCG35750.091.0077445
TGAGGTA60050.090.169383
TCGTGTT34050.087.747794
ACTACGG12650.087.732399
AGTGTCG29900.086.475997
TAGTCGA15850.086.0568544
CCGATGT26850.082.688733
GCCCGAT26800.082.430221
CCACGTT41350.081.68242
CGTCTCC85400.080.785731
CTAGTCG17000.079.888023
CGATGTG27850.079.719664
ACGTTGC40750.079.406434
CACGGGT20150.079.100889
TCCTCGC87750.079.066685
Produced by FastQC (version 0.11.5)