FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790751

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790751
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4143518
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGCCAAGACTTCGGGGGGGGGGGGGGGGGGGGGGGGGGG166340.4014463072200965No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGATT7800.078.6920859
GCGAATT10000.076.8863149
CCGCCTA17250.076.786097
ACGTATA9200.076.549219
ATACCGC14950.076.50354
ACGTTAT4950.074.3997968
CGCCTAA17400.073.522128
CGTTATA9950.072.727229
TACATTA39250.072.29724145
TCGCATA9700.069.939039
GCGGATT13500.068.43919
CGCGATT7750.068.3619169
GATACCG17450.066.6596453
CGCTATT10100.065.8897559
GCGCTAT6550.065.103558
CCGCATA11150.064.320619
ATCGATT9550.064.272079
GCGTATA11100.064.028279
GACGTAT4900.063.2917638
CCGTTAT6950.063.2158748
Produced by FastQC (version 0.11.5)