FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790752

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790752
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5354593
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA258210.4822215245864625No Hit
GCCACTTGATTTCTTTTCCTTTCTCTCTCTACCAATATTAAGCCTTAGTG204810.3824940569712768No Hit
AGATGTTTGGAGCTTTGGAGTGGTTCTGTATCNNN182650.3411090254665481No Hit
CTTTCGTGTTCAAACCACGGAGTTCACAACACAGCAGCACACACAGCCGG146140.27292457148470484No Hit
CAGCACATACACATTTTAGCATACCACAAATTCTTAACCCTTTCATATTC113660.21226636646333344No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAATTGCCT100690.18804417067739787No Hit
CTGTGTTCTTAGGCGGTATCACCNNNNNNNNNNNN98650.184234357307829No Hit
CCCACCCTAATGCCCTAATGTCNNNNNNNNNNNNN97620.1823107750673114No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAACTGTCT96510.1802377883809283No Hit
AGAAAGAATAAGCATGTTACCTTAAGATCAACCACCATGGACTGAACAAG78910.14736881029053No Hit
CGGCGGCTGGTAAGGGAAGAGGGGCTCCTCACTTCCCAGTAGGGGCGGCC78310.1462482769465392No Hit
TAGATTAAACACTCAACAAAGTAAACATGTTTCTGTCCTCTGTCTCATGT72830.13601407240475608No Hit
TCTCATACCTCCATTCCAAGCTTTCATNNNNNNNN60520.11302446329721046No Hit
CCTGAAGCCACGGGTGTCTTAAGGCTTCTTCTGTCATAAAACGTGCCTTT59390.11091412549936101No Hit
TCCACGTTGCGCTGCTTGGCGCGGATCGCACGCAGGAACCATGTGATGTC59220.11059664105189694No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGAGT3400.098.65093145
TAGCGGC34250.095.2842568
GCTAGCC39550.094.797251
TTAGGCG18200.091.1313559
TAGTCGA16900.090.756734
CGTTGCG24900.084.367615
TCGTGTT36300.083.35544
GCCCGAT26700.083.179121
GGTAGCG40000.081.4638756
GTAGCGG40950.079.694417
CTAGTCG19500.078.961883
CGATACT4850.077.498497
CGCACGT9100.077.4146651
CGGGTTA5250.076.163685
ACCCTAA21100.076.0855564
TGACCGA28950.075.427029
CCGATGT30000.074.600623
AGTGTCG28800.074.16267
CGTGTTC40850.074.071025
CGATGTG30200.073.908964
Produced by FastQC (version 0.11.5)