FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790753

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790753
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5354593
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGCCAAGACTTCGGGGGGGGGGGGGGGGGGGGGGGGGGG195600.3652938701410173No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAATT11800.082.660339
CGCGATT10500.076.173469
ACGTATA12200.075.686159
ATCGATT10200.074.588799
GACGTAT6200.073.416598
AGCGATA14950.073.072849
CGCCTAA21700.072.517628
ACCGATA12700.072.194369
CCGCCTA20250.070.6463247
CGTGATT9250.070.2985159
ATACCGC17850.069.947994
ACGTTAT8650.068.408988
CCGCATA13650.067.6462259
CGCGATA11950.066.930659
GATACCG19100.064.0101553
CTCGATA14000.063.632719
ACGCGAT9050.063.2298248
CGCTATT13100.061.5514459
GCGCTAT7000.061.310358
CGTTATA13000.061.0245179
Produced by FastQC (version 0.11.5)