FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790756

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790756
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5661740
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA197830.349415550696429No Hit
CCCACCCTAATGCCCTAATGTCNNNNNNNNNNNNN141830.25050602818214895No Hit
TCCACGTTGCGCTGCTTGGCGCGGATCGCACGCAGGAACCATGTGATGTC141440.2498171940074959No Hit
GCCACTTGATTTCTTTTCCTTTCTCTCTCTACCAATATTAAGCCTTAGTG134660.23784207681737415No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAACTGTCT127380.22498383889051776No Hit
CTTTCGTGTTCAAACCACGGAGTTCACAACACAGCAGCACACACAGCCGG116250.2053255712908046No Hit
GCCGCCAAGGAGCGGGAGATCCATCGCAAGCTGCAAGCGTTCTGCGCAAG104860.1852080809079894No Hit
TCTCATACCTCCATTCCAAGCTTTCATNNNNNNNN100570.17763090498680617No Hit
AGAAAGAATAAGCATGTTACCTTAAGATCAACCACCATGGACTGAACAAG98290.1736038744272962No Hit
GGGCATCGGCATCGGCATGTCGGCGCGGTTCGCCGAGGTGTCGATGATGC94300.16655657094815374No Hit
CGGCGGCTGGTAAGGGAAGAGGGCATCTTCTGCGGCGTTTCGTCTGGCGG91700.16196434311713362No Hit
CGTCTCCTCGCCGCGATCCTGGAGCGTGAGGTCGCCGCCAGGAACGCCTC78650.1389148918883594No Hit
CAGCACATACACATTTTAGCATACCACAAATTCTTAACCCTTTCATATTC67570.11934493636231971No Hit
CGTCTCCTCGCCGCGATCCTCGAGCCGTCCGCCCTCGGCGTCATCACCAT65030.11485868301970771No Hit
CTGTGTTCTTAGGCGGTATCACCNNNNNNNNNNNN63840.11275685566627927No Hit
AGATGTTTGGAGCTTTGGAGTGGTTCTGTATCNNN63650.11242126978632011No Hit
CGGCGGCTGGTAAGGGAAGAGGGGCTCCTCACTTCCCAGTAGGGGCGGCC59190.10454383281464708No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGGC54400.0100.278788
CGTTGCG44350.092.887755
GTAGCGG61800.088.3674557
TAGTCGA19050.088.310084
GGTAGCG62300.087.593826
CGCGTGT2900.086.88334145
TGAGGTA63400.086.261583
AGTGTCG36750.084.8921057
GCTAGCC34400.084.01151
CCACGTT50050.082.786412
ACTACGG13100.081.651959
ACCCTAA27600.081.629844
ACGTTGC50700.080.784894
CGTCTCC102250.080.488471
TCGTGTT37700.080.10164
GCCCGAT29800.079.619151
GTTGCGC51800.079.413656
TTGCGCT52000.079.080257
TTAGGCG15950.078.984259
TCCTCGC105750.078.586085
Produced by FastQC (version 0.11.5)