FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005790764

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790764
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7343502
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA363070.4944098878164669No Hit
AGATGTTTGGAGCTTTGGAGTGGTTCTGTATCNNN177790.24210519722061763No Hit
GCCACTTGATTTCTTTTCCTTTCTCTCTCTACCAATATTAAGCCTTAGTG155370.211574804500632No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAACTGTCT151510.20631845677988514No Hit
CGGCGGCTGGTAAGGGAAGAGGGGCTCCTCACTTCCCAGTAGGGGCGGCC151180.2058690799022047No Hit
AGAAAGAATAAGCATGTTACCTTAAGATCAACCACCATGGACTGAACAAG138680.18884722847491564No Hit
CTTTCGTGTTCAAACCACGGAGTTCACAACACAGCAGCACACACAGCCGG126680.17250625110471818No Hit
TACTAGTCGAAAGCGGCCCCGTGAAGGGGAAGCCGAGGGTGCCGAGACCA85230.11606179177182767No Hit
CTGACGGCCATGTCTTCCTTTGGCCACAGGACGCTGTGCGGAGCTGCAAG80240.10926666868205387No Hit
CTGGGCTCATGTGACCTACTGCCTTGGCCTCCCAAAGTGCTGGGATTACA75660.10302986231909518No Hit
CAAACAGACGCTGGGCTATCGAAGCCCCGACGCCGGGCCACCGCACGCTT75570.10290730498881868No Hit
CAAAAGGAGAGCGTATCCAAGAGGCCTAGCAAGAGAATGAGAATCCGCAT74400.10131405969522445No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)