FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790767

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790767
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15973623
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGCCAAGACTTCGGGGGGGGGGGGGGGGGGGGGGGGGGG407860.25533343312284257No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT26600.080.958629
GCGAATT36350.072.9555749
ACGTATA36400.072.67989
GATCGAT20650.070.2445148
ACCGATA36300.070.239449
TACATTA180450.068.119644145
GACGTAT18250.067.577338
TCGCATA34100.067.462579
CGTGATT27600.066.448879
CCGCATA37650.065.8539059
GCGCATA37000.065.801849
AGCGATA49400.063.2552649
ACGCATT34000.061.4587339
CGAGGAT23550.061.3231058
CGGTATA36500.060.750719
CGACATT30050.059.3300259
CGTAATT47700.059.213229
CGCTATT40000.058.470379
ATCGATT39600.058.4155129
ACGTTAT23900.058.0187648
Produced by FastQC (version 0.11.5)