FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790770

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790770
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4661095
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTGTTCTTAGGCGGTATCACCNNNNNNNNNNNN587921.261334514743853No Hit
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA230800.49516261736780737No Hit
CGGCGGCTGGTAAGGGAAGAGGGGCTCCTCACTTCCCAGTAGGGGCGGCC122990.263865036005488No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN116780.2505419863787372No Hit
CCCACCCTAATGCCCTAATGTCNNNNNNNNNNNNN107080.22973142577012484No Hit
GCCACTTGATTTCTTTTCCTTTCTCTCTCTACCAATATTAAGCCTTAGTG107070.2297099715839304No Hit
AGATGTTTGGAGCTTTGGAGTGGTTCTGTATCNNN102910.22078503012704095No Hit
TACTAGTCGAAAGCGGCCCCGTGAAGGGGAAGCCGAGGGTGCCGAGACCA94000.2016693502277898No Hit
CTTTCGTGTTCAAACCACGGAGTTCACAACACAGCAGCACACACAGCCGG91220.1957050864657339No Hit
AGAAAGAATAAGCATGTTACCTTAAGATCAACCACCATGGACTGAACAAG71480.15335452291789806No Hit
CCTGAAGCCACGGGTGTCTTAAGGCTTCTTCTGTCATAAAACGTGCCTTT65580.14069655306317505No Hit
CGCGTGCCCGTCTCGACTCTTATACACATCTCCGAGCCCACGAGACCGAT60950.1307632648551467No Hit
ACAGAGTCTGGCCATGTTACCCAATCTGATCTTGAACTCCTGCTGTCATC54800.11756894034556259No Hit
CAGCTAACATCATACTTAGACTGCAAACTGGCCGGGGATGGTGCCTCACA51530.11055342145997883No Hit
CTGGGCTCATGTGACCTACTGCCTTGGCCTCCCAAAGTGCTGGGATTACA51260.1099741584327288No Hit
AACAGAAACAAGAACTTCAGGCGCCAAGTAGGTGGGGGTTCCACATAAGG51060.10954507470883987No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGCG74550.0108.618729
TAGTCGA30650.0107.6597064
CGTGAGT2700.0100.183815145
CTAGTCG32700.0100.041983
CTTAGGC83850.096.503848
TGACCGA35250.092.144579
TAGCGGC25350.091.617448
CGCACGT8600.089.8322141
AGTCGAA36750.089.171485
ACTAGTC36950.088.8415762
TCGTGTT28050.085.445094
GCTAGCC39800.085.208491
CGTCGGA13600.085.1925
CGATGTG38750.084.422524
GCCCGAT39300.082.8235241
GTTCTTA97050.082.748755
CCGATGT40050.082.107643
TGTCGAC16700.079.906889
ACCCTAA20950.079.4312064
GCTAGTC4450.079.129653
Produced by FastQC (version 0.11.5)