FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790772

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790772
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8487535
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA409680.4826843129365593No Hit
GCCACTTGATTTCTTTTCCTTTCTCTCTCTACCAATATTAAGCCTTAGTG192110.22634369107167157No Hit
AGAAAGAATAAGCATGTTACCTTAAGATCAACCACCATGGACTGAACAAG179260.21120384186928245No Hit
CGGCGGCTGGTAAGGGAAGAGGGGCTCCTCACTTCCCAGTAGGGGCGGCC173510.20442920117560637No Hit
CTTTCGTGTTCAAACCACGGAGTTCACAACACAGCAGCACACACAGCCGG156640.18455299447955148No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAACTGTCT154400.18191383010497159No Hit
CCTGAAGCCACGGGTGTCTTAAGGCTTCTTCTGTCATAAAACGTGCCTTT124640.14685064627126723No Hit
TACTAGTCGAAAGCGGCCCCGTGAAGGGGAAGCCGAGGGTGCCGAGACCA98960.11659451183411909No Hit
CTGGGCTCATGTGACCTACTGCCTTGGCCTCCCAAAGTGCTGGGATTACA93220.10983165312425809No Hit
CATTTCTCTCATTAGCCCGGTTTTCAGATACTCAATACCAAAGAATTGAA92430.1089008764028661No Hit
CTGACGGCCATGTCTTCCTTTGGCCACAGGACGCTGTGCGGAGCTGCAAG91490.10779336992424773No Hit
CAATGACAGCAGCTGACTTGACTTTGCTAATGCCAAACAGCAAGTAAAAT88700.10450619643983794No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCGA26100.0107.399044
CGACGTA10950.0100.6902855
TAGCGGC25200.094.906338
CGCACGT12900.094.187031
GCTAGCC67000.092.6255341
TCGTGTT55900.087.25264
TGACCGA38250.086.1367959
CGGTTTA10050.085.557721
TGTCGAC27250.085.480999
CACGGGT42000.084.970829
CGTGTTC58500.083.374715
CTAGTCG33700.082.808813
AGTCGAA34400.081.848065
CCGATGT40650.081.829353
CGATACT11200.081.189397
GCCCGAT41700.079.939951
CGTCGGA23400.079.594635
CGTGAGT6000.079.0764145
GTTGCGA12000.078.383416
CGCGTGT4500.078.100136145
Produced by FastQC (version 0.11.5)